CAJ | 학술논문

利用PCR方法克隆SMYD3两个亚型的启动子区域，并构建其荧光素酶报告质粒。转染COS-7细胞后，利用荧光素酶报告分析技术，对二者启动子的转录活性进行了检测和比较。结果显示：SMYD3亚型1启动子的转录活性要显著高于亚型2启动子，生物信息学分析提示其原因可能与含有转录因子E2F-1结合位点的串联重复序列有关。
이용PCR방법극륭SMYD3량개아형적계동자구역，병구건기형광소매보고질립。전염COS-7세포후，이용형광소매보고분석기술，대이자계동자적전록활성진행료검측화비교。결과현시：SMYD3아형1계동자적전록활성요현저고우아형2계동자，생물신식학분석제시기원인가능여함유전록인자E2F-1결합위점적천련중복서렬유관。
In this study,promoters of two isoforms were cloned with PCR method and then their luciferase reporter plas-mids were constructed. Using the method of Luciferase Assay,the transcriptional activities of these two promotors of SMYD3 were detected in COS-7 cells. Results showed that the transcriptional activity of the promoter of isoform 1 is higher that that of the promoter of isoform 2. Bioinformatics analyses indicated that the significant difference in the transcriptional activity between these two promoters might be due to the tandem repeats of E2F-1 binding sites located in the promoter of isoform 1.