商洛学院学报
商洛學院學報
상락학원학보
JOURNAL OF SHANGLUO UNIVERSITY
2014年
4期
3-7,18
,共6页
铜胁迫%小麦幼苗%抗氧化系统
銅脅迫%小麥幼苗%抗氧化繫統
동협박%소맥유묘%항양화계통
copper stress%wheat seedlings%antioxidant systems
押以小麦种子为材料,通过液体培养实验探究铜对小麦幼苗生长和抗氧化系统的响应,揭示小麦幼苗对铜胁迫的生理抗性机制。结果表明,低浓度铜对小麦幼苗生长具有一定的促进作用,随着铜浓度的进一步增大和培养时间的延长,小麦幼苗的生长量明显减少,根和叶片中的膜脂过氧化产物丙二醛(MDA)、超氧化物歧化酶( SOD)、过氧化物酶( POD)、过氧化氢酶( CAT)和脯氨酸( Pro)的含量均明显升高,与对照相比达到5%或1%的差异显著性水平,这些活性物质在根和叶中的变化趋势基本一致,其含量除POD在根中分布大于叶中外,其余均表现为在叶中的分布大于在根中的分布。在过量Cu2+引起胁迫的过程中,抗氧化系统各成分通过相互协同配合,对铜胁迫进行有效的抵御,对自身进行严密的保护。
押以小麥種子為材料,通過液體培養實驗探究銅對小麥幼苗生長和抗氧化繫統的響應,揭示小麥幼苗對銅脅迫的生理抗性機製。結果錶明,低濃度銅對小麥幼苗生長具有一定的促進作用,隨著銅濃度的進一步增大和培養時間的延長,小麥幼苗的生長量明顯減少,根和葉片中的膜脂過氧化產物丙二醛(MDA)、超氧化物歧化酶( SOD)、過氧化物酶( POD)、過氧化氫酶( CAT)和脯氨痠( Pro)的含量均明顯升高,與對照相比達到5%或1%的差異顯著性水平,這些活性物質在根和葉中的變化趨勢基本一緻,其含量除POD在根中分佈大于葉中外,其餘均錶現為在葉中的分佈大于在根中的分佈。在過量Cu2+引起脅迫的過程中,抗氧化繫統各成分通過相互協同配閤,對銅脅迫進行有效的牴禦,對自身進行嚴密的保護。
압이소맥충자위재료,통과액체배양실험탐구동대소맥유묘생장화항양화계통적향응,게시소맥유묘대동협박적생리항성궤제。결과표명,저농도동대소맥유묘생장구유일정적촉진작용,수착동농도적진일보증대화배양시간적연장,소맥유묘적생장량명현감소,근화협편중적막지과양화산물병이철(MDA)、초양화물기화매( SOD)、과양화물매( POD)、과양화경매( CAT)화포안산( Pro)적함량균명현승고,여대조상비체도5%혹1%적차이현저성수평,저사활성물질재근화협중적변화추세기본일치,기함량제POD재근중분포대우협중외,기여균표현위재협중적분포대우재근중적분포。재과량Cu2+인기협박적과정중,항양화계통각성분통과상호협동배합,대동협박진행유효적저어,대자신진행엄밀적보호。
with wheat seeds as experimen tal material and through liquid culture experiment, the study explored the effect of copper stress on the growth and antioxidant system of wheat seedlings and revealed the physiological mechanism of resistance to that stress. The results showed that low concentration of copper had a certain role in promoting the growth of wheat seedlings, but with the further increase of Cu concentration and prolongation of culture time, the growth of wheat seedlings decreased signifcantly, while the content of malondialdehyde (MDA) resorted from membrane lipid peroxidation, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and proline (Pro) increased significantly and the content difference reached significant level (5% or 1% ). The changing trend of these active substances are basically the same in roots and leaves of the wheat seedlings, except the content of POD in roots is more than that in leaves, the rest of active substances distributed in leaves were more than that in roots.In the process of the stress caused by excessive Cu2+, antioxidant system together with each other to alleviate the toxic effects of oxidative stress caused by excess copper and protect plant closely.