CAJ | 학술논문

目的：研究VEGF-D在口腔癌相关成纤维细胞( carcinoma-associated fibroblasts,CAFs)的表达情况以及其表达水平是否与口腔鳞癌淋巴结转移相关。方法：通过免疫组化和RT-PCR检测VEGF-D在NFs、无淋巴结转移CAFs、淋巴结转移CAFs的蛋白和mRNA表达情况。结果：成功培养和纯化CAFs,与NFs相比,CAFs阳性表达α-SMA,生长增殖速度明显增加,细胞排列混乱；VEGF-D在淋巴结转移 CAFs的染色强度明显增加,用2-ΔΔCT 法计算 VEGF-D 的 mRNA 表达水平分别为0．8067±0.117、1.1925±0.125、2.0853±0.131。结论：与NFs相比,CAFs表达VEGF-D明显增加,且与患者淋巴结转移相关。
목적：연구VEGF-D재구강암상관성섬유세포( carcinoma-associated fibroblasts,CAFs)적표체정황이급기표체수평시부여구강린암림파결전이상관。방법：통과면역조화화RT-PCR검측VEGF-D재NFs、무림파결전이CAFs、림파결전이CAFs적단백화mRNA표체정황。결과：성공배양화순화CAFs,여NFs상비,CAFs양성표체α-SMA,생장증식속도명현증가,세포배렬혼란；VEGF-D재림파결전이 CAFs적염색강도명현증가,용2-ΔΔCT 법계산 VEGF-D 적 mRNA 표체수평분별위0．8067±0.117、1.1925±0.125、2.0853±0.131。결론：여NFs상비,CAFs표체VEGF-D명현증가,차여환자림파결전이상관。
Objective:To explore the protein expression level of VEGF-D in oral carcinoma-associated fibroblasts and analyze its cor-relation with lymphatic metastasis of oral carcinoma. Methods:The protein and mRNA expression levels of VEGF-D in NFs,Non-meta-static CAFs and metastatic CAFs were detected by immunochemical staining and RT-PCR after digesting the primary culture human mu-cosa cells. Results:We successfully cultured and purified CAFs. The CAFs were positive byα-SMA Immunohistochemical staining,high proliferation rate,chaos cytoarchitecture and the staining intensity of VEGF-D.β-actinas used as a reference gene,the mRNA level of VEGF-D respectively showed 0. 8,1. 2 and 2. 1. Conclusion:Compared with the NFs,CAFs increased the expression of VEGF-D,and the expression levels of VEGF-D correlates with the lymphatic metastasis.