渔业科学进展
漁業科學進展
어업과학진전
MARINE FISHERIES RESEARCH
2014年
4期
103-109
,共7页
海洋微生物%过氧化氢酶%不动杆菌属%鉴定%培养条件优化
海洋微生物%過氧化氫酶%不動桿菌屬%鑒定%培養條件優化
해양미생물%과양화경매%불동간균속%감정%배양조건우화
Marine microorganism%Catalase%Acinetobacter%Identification%Culture optimization
对来自青岛近海海域底泥的一株产过氧化氢酶菌株YS0810进行形态学观察、16S rDNA序列同源性分析及生理生化特性的鉴定，在250 ml摇瓶中进行发酵产酶条件优化。初步确定该菌属于不动杆菌属Acinetobacter。发酵培养的最佳碳、氮源分别为蔗糖20 g/L和蛋白胨15 g/L，无机盐MgSO4·7H2O、NaCl、KH2PO4最佳浓度分别为0.9、5.0和1.0 g/L；菌株在培养基起始pH=7.0、4%接种量、50 ml装液量和25℃的条件下发酵24 h获得较高的酶产量。在最佳培养条件下酶产量为2469 U/ml，是优化前的5倍。
對來自青島近海海域底泥的一株產過氧化氫酶菌株YS0810進行形態學觀察、16S rDNA序列同源性分析及生理生化特性的鑒定，在250 ml搖瓶中進行髮酵產酶條件優化。初步確定該菌屬于不動桿菌屬Acinetobacter。髮酵培養的最佳碳、氮源分彆為蔗糖20 g/L和蛋白胨15 g/L，無機鹽MgSO4·7H2O、NaCl、KH2PO4最佳濃度分彆為0.9、5.0和1.0 g/L；菌株在培養基起始pH=7.0、4%接種量、50 ml裝液量和25℃的條件下髮酵24 h穫得較高的酶產量。在最佳培養條件下酶產量為2469 U/ml，是優化前的5倍。
대래자청도근해해역저니적일주산과양화경매균주YS0810진행형태학관찰、16S rDNA서렬동원성분석급생리생화특성적감정，재250 ml요병중진행발효산매조건우화。초보학정해균속우불동간균속Acinetobacter。발효배양적최가탄、담원분별위자당20 g/L화단백동15 g/L，무궤염MgSO4·7H2O、NaCl、KH2PO4최가농도분별위0.9、5.0화1.0 g/L；균주재배양기기시pH=7.0、4%접충량、50 ml장액량화25℃적조건하발효24 h획득교고적매산량。재최가배양조건하매산량위2469 U/ml，시우화전적5배。
Catalases are a type of enzymes that can effectively decompose hydrogen peroxide into water and oxygen. Because of their ubiquitous distribution in all aerobic microorganisms, plants and animals, they are widely used in food, pharmaceutical, chemical industries and environmental protection. In this study we examined the bacterial strain YS0810 collected from the sediment in Yellow Sea that produces catalase at a high level. We determined the growth conditions of this strain which is optimal for the catalase production. Phylogenetic analysis of the 16S rDNA of this strain was applied to determine its taxonomic rank. The conventional morphological, physiological and biochemical methods of taxonomy were also applied to differentiate the YS0810 from its phylogenetic relatives. We used 250 ml shake flasks to carry out the single factor experiments to identify the important growth factors for the strain YS0810. The phylogenetic tree indicated that strain YS0810 belonged to the genusAcinetobacter, with the highest sequence similarity toAcinetobacter haemolyticus DSM 6962T (98.7%). However, YS0810 was not a strain ofA. haemolyticus. This was consistent with the results of the comparison between YS0810 and otherAcinetobacterspecies in terms of their morphological, physiological and biochemical characteristics. Peptone and sucrose were determined as the optimal nitrogen and carbon sources from several candidates. Our experimental results indicated that the maximum yield of the catalase was generated by YS0810 under the conditions shown below: peptone 15 g/L, sucrose 20 g/L, MgSO4·7H2O 0.9 g/L, NaCl 5.0 g/L, KH2PO4 1.0 g/L, broth content at 50 ml, the inoculum at 4%, initial pH 7.0, the temperature at 25°C and the culture time of 24 h. The verification experiment carried out in the optimal conditions above showed that the highest catalase activity was 2469 U/ml, which is five-time higher than before the optimization. In conclusion, the yield of catalase was markedly raised in the optimized fermentation conditions.
