CAJ | 학술논문

利用配置连续排污系统的室外水泥池养殖凡纳滨对虾(Litopenaeus vannamei)2012级育种核心群家系，监测、分析3个生长阶段养殖环境的水质变化、育种群体的生长和存活性能，为建立安全、高效的育种核心群体养殖模式提供基础数据。结果显示，在较高养殖密度(180 ind/m2)条件下，氨氮含量在7 d内从0达到1.80 mg/L的高峰值，在随后的养殖过程中呈波浪式变化，波动范围为0.50-1.80 mg/L；亚硝酸盐含量维持在低于0.60 mg/L的水平；pH值在7.2-8.2之间；溶藻弧菌、副溶血弧菌和哈维氏弧菌的含量分别为500-6200、0-400和0-10 CFU/ml；凡纳滨对虾的平均生长速率为0.199 g/d，养殖成活率达到89.7%；凡纳滨对虾体质量的生长符合Boltzmann和Logistic模型，体质量观测值与模型拟合值的决定系数(R2)分别为0.978和0.980，预测体质量规格为10-12 g时，为其体质量增长拐点。
이용배치련속배오계통적실외수니지양식범납빈대하(Litopenaeus vannamei)2012급육충핵심군가계，감측、분석3개생장계단양식배경적수질변화、육충군체적생장화존활성능，위건립안전、고효적육충핵심군체양식모식제공기출수거。결과현시，재교고양식밀도(180 ind/m2)조건하，안담함량재7 d내종0체도1.80 mg/L적고봉치，재수후적양식과정중정파랑식변화，파동범위위0.50-1.80 mg/L；아초산염함량유지재저우0.60 mg/L적수평；pH치재7.2-8.2지간；용조호균、부용혈호균화합유씨호균적함량분별위500-6200、0-400화0-10 CFU/ml；범납빈대하적평균생장속솔위0.199 g/d，양식성활솔체도89.7%；범납빈대하체질량적생장부합Boltzmann화Logistic모형，체질량관측치여모형의합치적결정계수(R2)분별위0.978화0.980，예측체질량규격위10-12 g시，위기체질량증장괴점。
In this study the nucleus families ofLitopenaeus vannameiproduced in 2012 were reared in the outdoor concrete tanks with continuous wastewater discharge. To establish a safe and efficient culture mode for the nucleus selection population, we analyzed their growth and survival at three growth stages in five concrete tanks with certain water qualities. Electric PVC butterfly valves installed in the center of the ponds were used to manage the timed water discharge. The shrimp were fed with commercial diets 4 times daily, and the wastewater was discharged 2 hours after the feeding. The concentrations of ammonia nitrogen and microorganism were monitored on daily and weekly basis respectively. The experiment lasted for 92 days. At every stage two ponds were in use except for the first one, which ended up dividing into two ponds. The shrimp were transferred into new ponds with freshwaters, and the survival rate was determined before entering into the next stage. Data collection, graphing, and the analysis of the growth model were done with Excell2010 and Origin 8.0. Under the high cultural density (180 ind/m2) the ammonia concentration increased to 1.80 mg/L after the first week, and ranged from 0.50 mg/L to 1.80 mg/L in the whole experimental period. The nitrite concentration maintained at a low level of 0.60 mg/L; the pH varied from 7.2 to 8.2; the range of concentrations ofV. alginolyticus, V. parahaemolyticus andV. harveyi were 500-6200 CFU/ml, 0-400 CFU/ml and 0-10 CFU/ml, respectively. During the three growth stages, the average growth and survival rate for the selected population were 0.199 g/d and 89.7% respectively. The growth of the selected population could fit in the Boltzmann and Logistic models. TheR2valuesof the actual and the predicted weights were 0.978 and 0.980 respectively. These results suggested that the inflection point of the growth was between 10 g and 12 g. The continuous wastewater discharge mode is viable and efficient in shrimp farming because of its low ammonia nitrogen toxicity, stable pH and low pathogenicity of the microorganisms.