CAJ | 학술논문

目的：应用MicroRNAs( miRNAs)芯片技术筛选鼻咽癌石蜡标本组织中差异表达的miRNAs,研究与鼻咽癌相关的miRNAs。方法从2例鼻咽癌石蜡切片和相配对的正常鼻组织中提取总RNA,miRNA芯片检测miRNA表达情况,然后在37例鼻咽癌石蜡切片和正常鼻组织中,用Real-time RT-PCR( Taqman)对部分差异表达miRNA进行验证。结果芯片结果显示：与配对的正常鼻组织相比,有24个miRNAs在鼻咽癌中异常表达,其中8个miRNA高表达,16个miRNA低表达。用Real-time RT-PCR( Taqman)验证显示,hsa-miR-205在鼻咽癌中表达水平升高( P=0.021)；hsa-miR-34c在鼻咽癌组织中表达水平降低( P=0.019)。结论 hsa-miR-205和hsa-miR-34c可能参与了鼻咽癌的发生过程。
목적：응용MicroRNAs( miRNAs)심편기술사선비인암석사표본조직중차이표체적miRNAs,연구여비인암상관적miRNAs。방법종2례비인암석사절편화상배대적정상비조직중제취총RNA,miRNA심편검측miRNA표체정황,연후재37례비인암석사절편화정상비조직중,용Real-time RT-PCR( Taqman)대부분차이표체miRNA진행험증。결과심편결과현시：여배대적정상비조직상비,유24개miRNAs재비인암중이상표체,기중8개miRNA고표체,16개miRNA저표체。용Real-time RT-PCR( Taqman)험증현시,hsa-miR-205재비인암중표체수평승고( P=0.021)；hsa-miR-34c재비인암조직중표체수평강저( P=0.019)。결론 hsa-miR-205화hsa-miR-34c가능삼여료비인암적발생과정。
Objective To screen aberrant miRNAs in nasopharyngeal carcinoma by miRNAs microarray. Methods Total RNA was extracted from nasopharyngeal cancer tissues and adjacent noncancerous tissues from paraffin sections. The miRNA expression profiles were analyzed using a mammalian miRNA microarray. Aberrant miRNAs were then verified by real-time RT-PCR in 37 nasopharyngeal cancer and nontumorous tissues. Results 24 aberrant miRNAs were identified by miRNA microarray, including 8-high expression of miRNAs and 16-low expression of miRNAs. Among them, the expression of hsa-miR-205 was verified to be higher( P=0.021) and hsa-miR-34c was identified to be significantly lower in the cancer tissues compared to the normal tissues(P=0.019). Conclusion hsa-miR-205 and hsa-miR-34c may be involved in the process of nasopharyngeal carcinogenesis.