CAJ | 학술논문

目的：优化胡芦巴多糖提取的最佳工艺。方法采用正交试验设计，以胡芦巴提取物出膏率及总多糖含量为评价指标，以苯酚-硫酸比色法测定胡芦巴中多糖的质量分数，考察提取时间、提取次数、固液比对胡芦巴中多糖提取效果的影响。结果影响胡芦巴多糖提取的因素大小依次为：提取次数>固液比>提取时间。优化得到最佳提取工艺为：加20倍体积的水，提取3次，每次1.5 h。结论本工艺合理、可行，适用于胡芦巴中多糖的提取。
목적：우화호호파다당제취적최가공예。방법채용정교시험설계，이호호파제취물출고솔급총다당함량위평개지표，이분분-류산비색법측정호호파중다당적질량분수，고찰제취시간、제취차수、고액비대호호파중다당제취효과적영향。결과영향호호파다당제취적인소대소의차위：제취차수>고액비>제취시간。우화득도최가제취공예위：가20배체적적수，제취3차，매차1.5 h。결론본공예합리、가행，괄용우호호파중다당적제취。
Objective To optimize the extraction process for the polysaccharides from Fenugreek. Methods The orthogonal design was used to evaluate the effects of extracting time,times and solid-liquid ratio with the yields of dry extract and polysaccharides as indexes. The phenol-sulfuric acid method was adopted to determine the polysaccharides. Results The results showed that the order of the selected factors influencing the extraction polysaccharides of fenugreek were as follows:extraction times>soild-liquid>extraction time. In the optional extraction process,the liquid-soild ration was 20∶1,extracting for 3 times and 1.5 h each time. Conclusion The extraction process was reasonable and feasible, which was suitable for the extraction of polysaccharides from Fenugreek.