CAJ | 학술논문

目的：建立葛根芩连滴丸的质量标准。方法采用薄层色谱法对滴丸中的葛根、黄芩、黄连进行定性鉴别；采用高效液相色谱法同时测定滴丸中的葛根素、盐酸小檗碱、黄芩苷。以甲醇-0.2%(体积分数)H3PO4(三乙胺调pH值至3.85)为流动相,梯度洗脱,流速为1.0 mL·min-1,检测波长为265 nm,柱温为35℃。结果定性鉴别分离度好,阴性无干扰；葛根素、盐酸小檗碱和黄芩苷的质量浓度分别在0．8175~13.08μg·mL-1( r=0.9999)、5.160~82.56μg·mL-1( r=0.9999)和12.39~198.2μg·mL-1( r=0.9998)范围内与相应峰面积呈良好线性关系,平均加样回收率(n=9)分别为101.6%(RSD=1．0%)、98.2%( RSD=0.6%)和100.6%( RSD=1.0%)。结论所建方法简单准确、重复性好,可用于葛根芩连滴丸的质量控制。
목적：건립갈근금련적환적질량표준。방법채용박층색보법대적환중적갈근、황금、황련진행정성감별；채용고효액상색보법동시측정적환중적갈근소、염산소벽감、황금감。이갑순-0.2%(체적분수)H3PO4(삼을알조pH치지3.85)위류동상,제도세탈,류속위1.0 mL·min-1,검측파장위265 nm,주온위35℃。결과정성감별분리도호,음성무간우；갈근소、염산소벽감화황금감적질량농도분별재0．8175~13.08μg·mL-1( r=0.9999)、5.160~82.56μg·mL-1( r=0.9999)화12.39~198.2μg·mL-1( r=0.9998)범위내여상응봉면적정량호선성관계,평균가양회수솔(n=9)분별위101.6%(RSD=1．0%)、98.2%( RSD=0.6%)화100.6%( RSD=1.0%)。결론소건방법간단준학、중복성호,가용우갈근금련적환적질량공제。
Objective To establish a method for the quality control of Gegen Qinlian dropping pills. Methods Puerariae lobatae Radix, Scutellariae Radix, Coptidis Rhizoma were identified by TLC and the contents of puerarin,berberine hydrochloride and baicalin were determined by HPLC. The mobile phase was consisted of methanol and 0.2% phosphoric acid( pH adjusted to 3.85 with triethylamine) at a flow rate of 1.0 mL·min-1 in a gradient elution. The detection wavelength was set at 265 nm and the column temperature was at 35 ℃. Results TLC identifications were performed with high resolution and without negative interference. The calibration curves were linear within the range of 0.817 5-13.08 μg·mL-1( r=0.999 9) for puerarin,5.160-82.56 μg·mL-1(r=0.999 9) for berberine hydrochloride and 12.39-198.2 μg·mL-1(r=0.999 8) for berberine,respectively. The average recoveries( n=9) were 101.6%( RSD=1.0%) for puerarin,98.2%( RSD=0.6%) for berberine hydrochloride and 100. 6%( RSD=1. 0%) for berberine, respectively. Conclusion The method was simple and accurate with a good reproducibility,and can be used for the quality control of Gegen Qinlian dropping pills.