CAJ | 학술논문

目的：研究药用植物广藿香内生真菌露湿漆斑菌( Myrothecium roridum)菌丝体的化学成分。方法采用正相硅胶柱层析、C18反相硅胶柱层析、sephadex LH-20凝胶柱层析以及薄层层析等色谱方法进行分离纯化，通过波谱数据分析结合文献对照对化合物结构进行鉴定。结果从广藿香内生真菌露湿漆斑菌菌丝体中分离并鉴定出5个化合物，分别为啤酒甾醇(1)、麦角甾醇-3-O-β-D-吡喃葡萄糖苷(2)、吡咯烷酮(3)、大黄酚(4)、尿嘧啶核苷(5)。结论化合物1~4均为首次从该属真菌中分离得到。
목적：연구약용식물엄곽향내생진균로습칠반균( Myrothecium roridum)균사체적화학성분。방법채용정상규효주층석、C18반상규효주층석、sephadex LH-20응효주층석이급박층층석등색보방법진행분리순화，통과파보수거분석결합문헌대조대화합물결구진행감정。결과종엄곽향내생진균로습칠반균균사체중분리병감정출5개화합물，분별위비주치순(1)、맥각치순-3-O-β-D-필남포도당감(2)、필각완동(3)、대황분(4)、뇨밀정핵감(5)。결론화합물1~4균위수차종해속진균중분리득도。
Objective To study on the chemical constituents of the mycelia of an endophytic Myrothecium roridum from Pogostemon cablin. (Blanco) Benth.. Methods The compounds were isolated and purified by various chromatographic methods such as silica gel, reverse phase silica gel, sephadex-LH20, preparative TLC,and so on. Their structures were determined by extensive analysis of their spectroscopic data as well as comparison with literature reports. Results Five compounds were isolated and identified as cerevisterol( 1) , ergosterol-3-O-β-D-glucopyranoside(2),pyrrolidone(3), chrysophanol(4) and uridine(5). Conclusion Compounds 1-4 are isolated from the genus Myrothecium for the first time.