CAJ | 학술논문

目的：建立同时测定大鼠血浆中隐丹参酮和丹参酮ⅡA浓度的反相高效液相色谱法,并对大鼠口服丹参脂溶性提取物后的2种丹参酮成分进行药动学研究。方法大鼠分别灌胃丹参脂溶性提取物混悬液(隐丹参酮和丹参酮ⅡA的灌胃剂量分别为38.4 mg·kg-1和70.1 mg·kg-1)后,眼眶静脉丛取血,经乙腈-乙酸乙酯(体积比1∶4)液-液萃取后采用HPLC法测定不同时间点的血药浓度,并用3P97软件计算药动学参数。结果隐丹参酮在0.05~5μg·mL-1内线性良好,方法回收率为99.7%~106．6%；丹参酮ⅡA在0.02~5μg·mL-1内线性良好,方法回收率为93.3%~102.5%。大鼠体内隐丹参酮和丹参酮ⅡA的t1/2(ka)分别为0.924、0.993 h,t1/2(ke)分别为2.632、2.126 h,Cmax分别为0.277、0．173μg·mL-1,AUC0-t分别为1.855、1.034μg·h·mL-1,CL/F(s)分别为20.700、67.763 mL·h-1·mg-1。结论建立的方法快速、简便、准确,可用于丹参脂溶性提取物中隐丹参酮和丹参酮ⅡA血浆样品的含量测定及药动学研究。
목적：건립동시측정대서혈장중은단삼동화단삼동ⅡA농도적반상고효액상색보법,병대대서구복단삼지용성제취물후적2충단삼동성분진행약동학연구。방법대서분별관위단삼지용성제취물혼현액(은단삼동화단삼동ⅡA적관위제량분별위38.4 mg·kg-1화70.1 mg·kg-1)후,안광정맥총취혈,경을정-을산을지(체적비1∶4)액-액췌취후채용HPLC법측정불동시간점적혈약농도,병용3P97연건계산약동학삼수。결과은단삼동재0.05~5μg·mL-1내선성량호,방법회수솔위99.7%~106．6%；단삼동ⅡA재0.02~5μg·mL-1내선성량호,방법회수솔위93.3%~102.5%。대서체내은단삼동화단삼동ⅡA적t1/2(ka)분별위0.924、0.993 h,t1/2(ke)분별위2.632、2.126 h,Cmax분별위0.277、0．173μg·mL-1,AUC0-t분별위1.855、1.034μg·h·mL-1,CL/F(s)분별위20.700、67.763 mL·h-1·mg-1。결론건립적방법쾌속、간편、준학,가용우단삼지용성제취물중은단삼동화단삼동ⅡA혈장양품적함량측정급약동학연구。
Objective To develop a reversed phase high-performance liquid chromatography for simultaneous determination cryptotanshinone and tanshinone ⅡA in rat plasma and to study on pharmacokinetics of the two tanshiones. Method The rats was administered the extract of Salvia miltiorrhiza ( 38. 4 mg · kg-1 cryptotanshinone and 70.1 mg·kg-1 tanshinone ⅡA), and the blood samples were collected from eye ophthalmic vein jungle venous plexus. After liquid liquid exatraction with acetonitrile-ethyl acetate, plasma concentrations of cryptotanshinone and tanshinone ⅡA were determined by HPLC. The 3P97 software package was used to calculate the pharmacokinetic parameters. Result A good linear relationship of cryptotanshinone was obtained from 0.05 to 5μg·mL-1 ,and the method recoveries were all from 99.7%-106.6%. A good linear relationship of tanshinone ⅡA was obtained from 0.02 to 5 μg·mL-1,and the method recoveries were all from 93.3%-102.5%. The pharmacokinetic parameters of cryptotanshinone and tanshinoneⅡA were as follows:t1/2(ka) was 0.924,0.993 h,t1/2(ke) was 2.632,2.126 h,Cmax was 0. 277,0.173μg·mL-1,AUC0-t was 1.855,1.034 μg·h·mL-1,CL/F(s) was 20.700,67.763 mL·h-1·mg-1. Conclusion The analysis method is rapid,simple and sensitive enough to be applied in the detection and pharmacokinetic study of cryptotanshinone and tanshinone ⅡA.