CAJ | 학술논문

冷激蛋白（ cold shock proteins Csps）作为细菌中RNA 的分子伴侣，含有原始型的冷休克结构域，具有与核酸结合功能，可以防止RNase对mRNA的降解，也能纠正mRNA的折叠错误，为了寻找作物改良的潜在基因资源，从耐辐射奇球菌（ Deinnoccus radiodurans DR1）基因组中克隆出csp2基因，利用农杆菌侵染法转化甘蓝型油菜，通过两代纯化得到T2代转基因油菜种子，以15%聚乙二醇溶液（ PEG6000）为渗透介质模拟干旱胁迫条件，对非转基因油菜和转csp2基因油菜种子萌发期抗旱性进行评价。结果显示：PEG胁迫下，转csp2基因油菜的发芽率、发芽势、发芽指数、活力指数，以及胚芽和培根的生长发育程度均高于非转基因油菜。从而表明，csp2基因能够促进植物细胞从逆境伤害中快速恢复。
랭격단백（ cold shock proteins Csps）작위세균중RNA 적분자반려，함유원시형적랭휴극결구역，구유여핵산결합공능，가이방지RNase대mRNA적강해，야능규정mRNA적절첩착오，위료심조작물개량적잠재기인자원，종내복사기구균（ Deinnoccus radiodurans DR1）기인조중극륭출csp2기인，이용농간균침염법전화감람형유채，통과량대순화득도T2대전기인유채충자，이15%취을이순용액（ PEG6000）위삼투개질모의간한협박조건，대비전기인유채화전csp2기인유채충자맹발기항한성진행평개。결과현시：PEG협박하，전csp2기인유채적발아솔、발아세、발아지수、활력지수，이급배아화배근적생장발육정도균고우비전기인유채。종이표명，csp2기인능구촉진식물세포종역경상해중쾌속회복。
Csp2,one of RNA chaperon in bacteria,has a prototypical cold shock domain( CSD)with a poly-nucleotide binding function which prevents mRNA from digestion by RNase and rescues misfolded mRNA. In search of candidate genes for crop improvement,csp2 gene was cloned from the genome of Deinnoccus radiodurans ( DR1 ). The purificated T2 generation of transgenic rapeseeds was got with the method of Agrobacterium-media-ted transform B. napus. The drought-resistance evaluation of transgenic and non-transgenic nape at seed ger-mination stage was studied by simulated drought stress with 15% PEG-6000 solution. The results showed that the growth and development of transgenic nape germination rate,germination energy,germination index,vitality index and the length of embryonic bud and embryonic root were higher than in non transgenic nape. To show that, csp2 gene under drought stress induced cold shock proteins,through the transcription and the readjustment of mo-lecular chaperone,and improve the ability turn csp2 gene rape of drought resistance.