CAJ | 학술논문

目的：通过进行金黄膏中小檗碱体外透皮吸收实验，探讨金黄膏中不同药对配伍[1]对其透皮效果的影响，进一步探讨其影响机制。方法采用体外经皮渗透法，使用透皮扩散试验仪，选择大鼠腹部皮肤为渗透屏障，以30％乙醇-磷酸盐缓冲溶液（ pH＝6．8）作为接受液，用高效液相色谱法测定接受液中小檗碱含量。考察全方药组及缺姜黄、白芷组（缺药方A组）和缺姜黄、白芷、天南星、陈皮、厚朴组（缺药方B组）中小檗碱的透皮特性。结果（1）缺药方A组和缺药方B组的稳态透皮速率、透皮时滞、32小时内小檗碱单位面积累积透过量都较全方高。（2）金黄散全方组的稳态透皮速率及32小时内盐酸小檗碱累积透过量都低于缺姜黄、白芷的缺药方A组，说明姜黄、白芷对小檗碱的皮肤渗透有抑制作用。（3）有天南星、陈皮、厚朴的缺药方A组的小檗碱透过量高，而透皮时滞低于缺药方 B组，说明天南星、陈皮、厚朴对小檗碱的皮肤渗透有促进作用。结论金黄膏中的姜黄、白芷和天南星、陈皮、厚朴这两组药都对金黄膏发挥疗效起到了促进作用。金黄膏中每组药对都各负其责，又相互协同，合理配伍组成一个整体的透皮系统。
목적：통과진행금황고중소벽감체외투피흡수실험，탐토금황고중불동약대배오[1]대기투피효과적영향，진일보탐토기영향궤제。방법채용체외경피삼투법，사용투피확산시험의，선택대서복부피부위삼투병장，이30％을순-린산염완충용액（ pH＝6．8）작위접수액，용고효액상색보법측정접수액중소벽감함량。고찰전방약조급결강황、백지조（결약방A조）화결강황、백지、천남성、진피、후박조（결약방B조）중소벽감적투피특성。결과（1）결약방A조화결약방B조적은태투피속솔、투피시체、32소시내소벽감단위면적루적투과량도교전방고。（2）금황산전방조적은태투피속솔급32소시내염산소벽감루적투과량도저우결강황、백지적결약방A조，설명강황、백지대소벽감적피부삼투유억제작용。（3）유천남성、진피、후박적결약방A조적소벽감투과량고，이투피시체저우결약방 B조，설명천남성、진피、후박대소벽감적피부삼투유촉진작용。결론금황고중적강황、백지화천남성、진피、후박저량조약도대금황고발휘료효기도료촉진작용。금황고중매조약대도각부기책，우상호협동，합리배오조성일개정체적투피계통。
Objective By performing berberine in vitro percutaneous absorption experiments to ex-plore the transdermal effect of the different compatibility of drugs in the Jinhuang Cream [1] and then to fur-ther explore the influencing mechanism .Methods In this study, in vitro percutaneous penetration method was used and transdermal diffusion test instrument was applied .Rat abdominal skin was selected as perme-ability barrier and 30% ethanol-phosphate buffer solution ( pH=6.8) was chosen as the accepted solu-tion..The concentration of berberine in the accepted solution was measured by HPLC method to examine the transdermal properties of the whole prescription , the lack of Turmeric , Angelica dahurica group ( lack of prescription group A) and the lack of Turmeric, Angelica dahurica, Araceae, Citrus, Magnolia group ( lack of prescription group B ) drugs.Results (1) Compared with the whole prescription group , the in-dexes of steady-state permeation rate, transdermal delay, 32h accumulated berberine unit area by volume and permeability were much highert in Lack of prescription group A and lack of prescription group B .(2) The steady state permeation rate and 32h accumulated berberine unit area by volume and permeability in the whole prescription group were both lower than that in the the lack prescription group A , which demon-strated that, Turmeric, Angelica dahurica can restrain the skin penetration of berberine .(3) The steady-state permeation rate in lack of prescription group A was higher than in lack of prescription group B , while and transdermal delay in lack prescription group A was lower than that in lack prescription group B .The results suggested that Araceae , Citrus, Magnolia can promote skin penetration of berberine .Conclusion Turmeric, Angelica dahurica and Araceae , Citrus, Magnolia both sets of the drugs in Jinhuang Cream can effective promote the percutaneous absorption of golden cream .Each drug in Jinhuang Cream plays an im-portant role on the compatibility , mutual collaboration and forming a whole transdermal system .