浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
15期
1321-1323,1327
,共4页
程翅%张海峰%闫康鹏%梁磊
程翅%張海峰%閆康鵬%樑磊
정시%장해봉%염강붕%량뢰
p27kip1%结肠癌%结肠肿瘤
p27kip1%結腸癌%結腸腫瘤
p27kip1%결장암%결장종류
p27kip1%Colon cancer%Immunohistochemical
目的：探讨p27kip1蛋白在结肠癌组织中的表达及意义。方法应用免疫组化方法检测结肠癌组织（观察组40例）及癌旁正常组织（对照组20例）中p27kip1蛋白的表达，比较其阳性表达率，并比较分析观察组不同分化程度及有无淋巴结转移患者p27kip1蛋白的阳性表达程度。结果观察组中p27kip1蛋白阳性表达16例（40.00%），对照组中p27kip1蛋白阳性表达15例（75.00%），差异有统计学意义（P＜0.05）。高、中分化患者p27kip1蛋白的阳性表达程度明显高于低分化患者，差异有统计学意义（P＜0.05）。有淋巴结转移患者p27kip1蛋白的阳性表达程度明显低于无淋巴结转移患者，差异有统计学意义（P＜0.05）。TNM分期Ⅰ～Ⅱ期患者p27kip1蛋白阳性表达12例（63.16%），Ⅲ～Ⅳ期患者p27kip1蛋白阳性表达4例（19.05%），差异有统计学意义（P＜0.05）。结论 p27kip1蛋白作为抑癌因子具有调控细胞增殖、分化及凋亡的能力，而组织中p27kip1蛋白的低表达可能导致了肿瘤的发生、发展。p27kip1蛋白可能作为临床上检测结肠癌的新指标，有助于结肠癌的肿瘤分化、肿瘤转移及肿瘤TNM分期的诊断。
目的：探討p27kip1蛋白在結腸癌組織中的錶達及意義。方法應用免疫組化方法檢測結腸癌組織（觀察組40例）及癌徬正常組織（對照組20例）中p27kip1蛋白的錶達，比較其暘性錶達率，併比較分析觀察組不同分化程度及有無淋巴結轉移患者p27kip1蛋白的暘性錶達程度。結果觀察組中p27kip1蛋白暘性錶達16例（40.00%），對照組中p27kip1蛋白暘性錶達15例（75.00%），差異有統計學意義（P＜0.05）。高、中分化患者p27kip1蛋白的暘性錶達程度明顯高于低分化患者，差異有統計學意義（P＜0.05）。有淋巴結轉移患者p27kip1蛋白的暘性錶達程度明顯低于無淋巴結轉移患者，差異有統計學意義（P＜0.05）。TNM分期Ⅰ～Ⅱ期患者p27kip1蛋白暘性錶達12例（63.16%），Ⅲ～Ⅳ期患者p27kip1蛋白暘性錶達4例（19.05%），差異有統計學意義（P＜0.05）。結論 p27kip1蛋白作為抑癌因子具有調控細胞增殖、分化及凋亡的能力，而組織中p27kip1蛋白的低錶達可能導緻瞭腫瘤的髮生、髮展。p27kip1蛋白可能作為臨床上檢測結腸癌的新指標，有助于結腸癌的腫瘤分化、腫瘤轉移及腫瘤TNM分期的診斷。
목적：탐토p27kip1단백재결장암조직중적표체급의의。방법응용면역조화방법검측결장암조직（관찰조40례）급암방정상조직（대조조20례）중p27kip1단백적표체，비교기양성표체솔，병비교분석관찰조불동분화정도급유무림파결전이환자p27kip1단백적양성표체정도。결과관찰조중p27kip1단백양성표체16례（40.00%），대조조중p27kip1단백양성표체15례（75.00%），차이유통계학의의（P＜0.05）。고、중분화환자p27kip1단백적양성표체정도명현고우저분화환자，차이유통계학의의（P＜0.05）。유림파결전이환자p27kip1단백적양성표체정도명현저우무림파결전이환자，차이유통계학의의（P＜0.05）。TNM분기Ⅰ～Ⅱ기환자p27kip1단백양성표체12례（63.16%），Ⅲ～Ⅳ기환자p27kip1단백양성표체4례（19.05%），차이유통계학의의（P＜0.05）。결론 p27kip1단백작위억암인자구유조공세포증식、분화급조망적능력，이조직중p27kip1단백적저표체가능도치료종류적발생、발전。p27kip1단백가능작위림상상검측결장암적신지표，유조우결장암적종류분화、종류전이급종류TNM분기적진단。
Objective To study the express of p27kip1 in carcinoma of colon,then to investigate its roles and probable mechanism in the pathogenesis of colon cancer. Methods Immunohistochemistry was used to detect the expression and distri-bution of p27kip1 in carcinoma of colon and normal colon. Statistics was used to analyze the data.Making a analysis of positive expression level of p27kip1 protein in colon caner tissue with different degree of differentiation and lymph node metastasis. Re-sults 16 cases of positive expression of p27kip1 Protein in observation group (40%), 15 cases of positive expression of p27kip1 Protein in the control group (75%), the difference was statistical y significant (P<0.05).The expression of p27kip1 Protein in group of high degree of differentiation was significantly higher than that group of low differentiated. The difference was statistical y sig-nificant (P<0.05). The positive expression of p27kip1 Protein in patients with the extent of lymph node metastasis was significantly lower than that in patients without lymph node metastasis, the difference was statistical y significant (P<0.05). 12 cases of positive expression of p27kip1 Protein in TNM stage I~II (63.16%), 4 cases of positive expression of p27kip1 Protein in TNM stageⅢ~Ⅳ(19.05%), the difference was statistical y significant (P<0.05). Conclusion p27kip1 protein as a tumor suppressor factor that can regulate cellproliferation, differentiation and apoptosis of ability. Low expression of p27kip1 protein led to tumor occurrence and development in organization.Protein of p27kip1 would be considered as new clinical index of colon carcinoma, and make for di-agnose of neoplasm differentiation, neoplasm metastasis and TNM staging.
