CAJ | 학술논문

对羟基红花黄色素A纯化进行了工艺研究。采用恒温水提法对红花进行浸提，X-5型吸附树脂分离，采用Sephadex LH-20柱层析对纯化工艺中的条件进行筛选，运用紫外分光光度计测定羟基红花黄色素A的含量。结果显示：纯化的最佳工艺条件为上样量为2％的柱体积，流速为1.0 mL/min，洗脱液浓度为50％的乙醇，径高比为1：8。采用紫外可见分光光度法测得羟基红花黄色素A的含量为86.4％。此方法具有操作方便，纯度较高的优点。
대간기홍화황색소A순화진행료공예연구。채용항온수제법대홍화진행침제，X-5형흡부수지분리，채용Sephadex LH-20주층석대순화공예중적조건진행사선，운용자외분광광도계측정간기홍화황색소A적함량。결과현시：순화적최가공예조건위상양량위2％적주체적，류속위1.0 mL/min，세탈액농도위50％적을순，경고비위1：8。채용자외가견분광광도법측득간기홍화황색소A적함량위86.4％。차방법구유조작방편，순도교고적우점。
The process of hydroxysafflor yellow A's purity was researched , using constant temperature water to extract of safflower.The way that use X -5 macroporous adsorption resin and Sephadex LH -20 column to purite , and the process conuditions of purification of sieve training , contented by ultraviolet visible spectrophotometry of hydroxysafflor yellow A's meter.The optimum conditions to purite was the sample volume must be 2%column volumes , and the flow rate was 1.0 mL/min, the eluent concentration of 50% ethanol, the diameter height ratio was 1:8.The content was determined by UV hydroxysafflor yellow.A spectrophotometry method was 86.4%.The advantages of the method were it had convenient operation and high purity.