CAJ | 학술논문

目的：探讨β-咔啉类生物碱对人胃癌细胞 SGC-7901增殖的影响。方法通过体外培养人胃癌细胞株 SGC-7901，将含有不同浓度（5、10、20、40μg/mL）β-咔啉类生物碱的培养液与 SGC-7901细胞共同培养24 h 和48 h。采用 MTT 比色法计算细胞抑制率；在荧光显微镜下用 Hoechst 33258细胞核染色法观察细胞形态学改变；流式细胞仪检测凋亡率及基因组DNA 琼脂凝胶电泳检测凋亡梯状条带。结果β-咔啉类生物碱对 SGC-7901细胞的损伤呈浓度依赖性；β-咔啉类生物碱分别作用24 h 和48 h 后半数抑制浓度（IC50）分别为17．79μg/mL 和12．17μg/mL；荧光染色可观察到有细胞核固缩及核断裂的凋亡现象；流式细胞术显示：阴性对照组（0μg/mL）及β-咔啉类生物碱5、10、20、40μg/mL 浓度组24、48 h 凋亡率为别1．66％、11．27％、20．32％、30．66％、41．42％和3．84％、15．29％、23．34％、34．87％、49．54％，细胞凋亡率伴随给药浓度增加而增加；基因组 DNA 琼脂糖凝胶电泳检测到明显的凋亡梯状条带。结论β-咔啉类生物碱能诱导人胃癌 SGC-7901细胞发生凋亡，抑制细胞增殖。
목적：탐토β-잡람류생물감대인위암세포 SGC-7901증식적영향。방법통과체외배양인위암세포주 SGC-7901，장함유불동농도（5、10、20、40μg/mL）β-잡람류생물감적배양액여 SGC-7901세포공동배양24 h 화48 h。채용 MTT 비색법계산세포억제솔；재형광현미경하용 Hoechst 33258세포핵염색법관찰세포형태학개변；류식세포의검측조망솔급기인조DNA 경지응효전영검측조망제상조대。결과β-잡람류생물감대 SGC-7901세포적손상정농도의뢰성；β-잡람류생물감분별작용24 h 화48 h 후반수억제농도（IC50）분별위17．79μg/mL 화12．17μg/mL；형광염색가관찰도유세포핵고축급핵단렬적조망현상；류식세포술현시：음성대조조（0μg/mL）급β-잡람류생물감5、10、20、40μg/mL 농도조24、48 h 조망솔위별1．66％、11．27％、20．32％、30．66％、41．42％화3．84％、15．29％、23．34％、34．87％、49．54％，세포조망솔반수급약농도증가이증가；기인조 DNA 경지당응효전영검측도명현적조망제상조대。결론β-잡람류생물감능유도인위암 SGC-7901세포발생조망，억제세포증식。
Objective To investigate the effects of β-Carboline of alkaloids on proliferation of SGC-7901 of human gastric cancer cells.Methods SGC-7901 cells were cultured in vitro initially. After SGC-7901 cells were incubated with β-Carboline alkaloids at different concentrations of 5,10, 20,40 μg/mL for 24,48 hours,the inhibited proliferation rate of SGC-7901 cells were examined by Methtl Thiazolyl Tetrazolium(MTT)assay.Morphological changes of SGC-7901 cells were observed by Hoechst 33258 under fluorescence microscope.Flow cytometry was used to detect cell apoptosis after SGC-7901 cells were incubated with β-Carboline alkaloids for 24 hours.Cell gemonic DNA was detec-ted by agarose electrophoresis.Results β-Carboline of alkaloids induced damage of SGC-7901 cell in a concentration dependent manner .The IC 5 0 of β-Carboline alkaloids at 2 4 ,4 8 hours were 17.79 μg/mL and 12.17 μg/mL respectively.Apoptotic cells were observed and flow cytonetry analy-sis in dicated that the apoptosis rate of cells treated with different concentrations of β-Carboline alka-loids(0,5,10,20,40 μg/mL)for 24 and 48 hours were 1.66%,11.27%,20.32%,30.66%, 41.42%;3.84%,15.29%,23.34%,34.87%,49.54%,respectively.Increasing in apoptosis rate of SGC-7901 cells was associated with drug concentration.Typical DNA Ladder was detected in DNA agarose electrophoresis.Conclusion β-Carboline alkaloids can inhibit the proliferation and in-duce the apoptosis of SGC-7901 cells.