CAJ | 학술논문

目的：利用高纯度的 rhuPAa-melittin 进行人成纤维细胞和卵巢癌细胞进行活性检测，探讨 rhuPAa-melittin 对卵巢癌治疗作用及对正常细胞毒性作用。方法利用高纯度的 rhuPAa-melittin 蛋白分别作用于人成纤维细胞和人卵巢癌细胞 SK-OV348 h，在490 nm 波长处测定其吸光度，以反映活细胞的数量并间接反映 rhuPAa-melittin 对培养中细胞的生长抑制作用。结果人卵巢癌细胞 SKOV3组，在4μg/mL 时对细胞抑制率已经达到了66．59％，在16μg/mL 时，细胞已经全部死亡，差异有统计学意义（P ＜0．05）。而人成纤维细胞组，在4μg/mL 时其对细胞的抑制率仅为达到7．3％，8μg/mL 时抑制率为12．3％，16μg/mL 时抑制率为22．0％。结论rhuPAa-melittin 对人卵巢癌细胞 SKOV3细胞有着明显的抑制杀伤作用，而对于人成纤维细胞的抑制作用不明显。
목적：이용고순도적 rhuPAa-melittin 진행인성섬유세포화란소암세포진행활성검측，탐토 rhuPAa-melittin 대란소암치료작용급대정상세포독성작용。방법이용고순도적 rhuPAa-melittin 단백분별작용우인성섬유세포화인란소암세포 SK-OV348 h，재490 nm 파장처측정기흡광도，이반영활세포적수량병간접반영 rhuPAa-melittin 대배양중세포적생장억제작용。결과인란소암세포 SKOV3조，재4μg/mL 시대세포억제솔이경체도료66．59％，재16μg/mL 시，세포이경전부사망，차이유통계학의의（P ＜0．05）。이인성섬유세포조，재4μg/mL 시기대세포적억제솔부위체도7．3％，8μg/mL 시억제솔위12．3％，16μg/mL 시억제솔위22．0％。결론rhuPAa-melittin 대인란소암세포 SKOV3세포유착명현적억제살상작용，이대우인성섬유세포적억제작용불명현。
Objective RhuPAa-melittin protein with high-purity was analyzed to investigate its biologic effect on normal and ovarian cancer cells.Methods Preliminary purified rhuPAa-melittin was added in medium normal cells and SKOV3 human ovarian cancer cells cultured for 48 hours,and then were measured at 490 nm wavelength absorbance to measure the number of living cells and evalu-ate the inhibitory effect of rhuPAa-melittin on cultured cells.Results The inhibitory rate of rhuPAa-melittin at 4 μg/mL was 66.59% in SKOV3 cancer cell progression.At a concentration of 16 μg/mL, rhuPAa-melittin killed all cancer cells (P <0.05).In normal adult fibrous cells,the inhibitory rate of rhuPAa-melittin at 4 μg /mL was only 7.3%,and 12.3% inhibition rate was obtained by using rhu-PAa-melittin (8 μg/mL).Moreover,inhibition rate topped at 22.0% when used rhuPAa-melittin at 16 μg/mL.Conclusion RhuPAa-melittin has a strong anti-tumor effect on SKOV3 cancer cells.