CAJ | 학술논문

目的：观察安徽省淮南市2012-2013年流感病毒细胞培养分离结果，研究淮南市流感流行特征，为该市流感防控提供科学依据。方法实时荧光定量PCR法对哨点医院送检咽拭子标本进行核酸快速分型后采用狗肾（ MDCK）传代细胞培养法分离病毒，红细胞凝集试验（ HA）鉴定病毒滴度，红细胞凝集抑制试验（ HI）进行型和亚型的鉴定。结果从148份流感网络实验室实时定量PCR阳性标本中，分离出流感毒株68株，总分离率45．9％，其中 A1（H1N1）型26株，分离成功率56．5％，A3（H3N2）型25株，分离成功率33.3％；BY型4株，BV型13株，B型毒株分离成功率为63．0％；各型毒株的分离成功率差异有统计学意义（P＜0．05）。2012年淮南市流行株为BV型流感病毒，于3月份达到流行高峰；2013年流行株为A（H1N1）型流感病毒，流行高峰期则是12月份。结论淮南市2012-2013年甲型流感病毒抗原的变异性强；细胞培养分离病毒对流感流行的病原鉴定起决定性作用；流感病毒B型毒株和新A（H1N1）型毒株分离成功率较高。
목적：관찰안휘성회남시2012-2013년류감병독세포배양분리결과，연구회남시류감류행특정，위해시류감방공제공과학의거。방법실시형광정량PCR법대초점의원송검인식자표본진행핵산쾌속분형후채용구신（ MDCK）전대세포배양법분리병독，홍세포응집시험（ HA）감정병독적도，홍세포응집억제시험（ HI）진행형화아형적감정。결과종148빈류감망락실험실실시정량PCR양성표본중，분리출류감독주68주，총분리솔45．9％，기중 A1（H1N1）형26주，분리성공솔56．5％，A3（H3N2）형25주，분리성공솔33.3％；BY형4주，BV형13주，B형독주분리성공솔위63．0％；각형독주적분리성공솔차이유통계학의의（P＜0．05）。2012년회남시류행주위BV형류감병독，우3월빈체도류행고봉；2013년류행주위A（H1N1）형류감병독，류행고봉기칙시12월빈。결론회남시2012-2013년갑형류감병독항원적변이성강；세포배양분리병독대류감류행적병원감정기결정성작용；류감병독B형독주화신A（H1N1）형독주분리성공솔교고。
Objective To reveal the epidemic characteristics of influenza in Huainan of Anhui Province and provide scientific basis for prevention and control of influenza surveillance by summarizing the work of influenza virus isolation performed in 2012-2013.Methods First nucleic acid fast typing by real-time PCR method for sentinel hospitals in throat swab specimens for inspection from sentinel hospital ,the influenza virus isolation was performed by using MDCK cells and the identification of the isolates was carried out by hemagglutination ( HA) and hemagglutination inhibition(HI) tests.Results 68 strains of influenza virus were isolated (45.9%) from 148 of positive specimens detected by fluorescent quantitative real-time PCR.The number of subtype A(H1N1),A(H3N2),BY and BV were 26 strains,25 strains,4 strains and 13 strains,respectively,the success rate of the separation were 56.5%,33.3%, 63.0%,respectively.There were significant difference among the separation of the strain rate of success (P<0.05). 2012 epidemic strain in Huainan was influenza BV virus ,and reached epidemic peak in March;2013 epidemic strain was A(H1N1)influenza virus,the epidemic peak was December.Conclusion During the period of influenza surveillance in Huainan,the main subtype of influenza of the year 2012 is BV which reached epidemic peak in March , while subtype A(H1N1) is the main subtype of the year 2013,with the epidemic peak month December .