中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
19期
2959-2960
,共2页
糖蛋白类%胆汁%胆道疾病%诊断,鉴别
糖蛋白類%膽汁%膽道疾病%診斷,鑒彆
당단백류%담즙%담도질병%진단,감별
Glycoproteins%Bile%Biliary tract diseases%Diagnosis,differential
目的：探讨胆汁糖蛋白糖链结构变化对于鉴别良恶性胆道疾病的作用。方法选取青岛市海慈医疗集团普外科收治的胆道疾病患者100例，按照良、恶性将其分为对照组（良性胆道疾病）和观察组（胆管癌），每组各50例。取两组患者胆汁滴于硝酸纤维膜上，通过比较麦胚凝集素（ WGA ）、欧曼陀罗凝集素（ DSA）、小扁豆凝集素（ LCA）、刀豆凝集素（ CONA）试验阳性率，探讨胆汁糖蛋白糖链结构变化与良恶性胆道疾病的关系。结果对照组 WGA、DSA、LCA、CONA 凝集素试验阳性率分别为22．0％、14．0％、2．0％、76.0％，观察组分别为76．0％、66．0％、76．0％、82．0％。两组CONA凝集素试验阳性率差异无统计学意义（P＞0．05），观察组WGA、LCA、DSA凝集试验阳性率均明显高于对照组，差异均有统计学意义（χ2＝29．17、28．17、57．55，均P＜0．05）。结论胆汁糖蛋白糖链结构变化与胆道疾病良、恶性密切相关，可以通过WGA、LCA、DSA凝集试验阳性率判断胆管疾病良、恶性，值得在临床上广泛推广。
目的：探討膽汁糖蛋白糖鏈結構變化對于鑒彆良噁性膽道疾病的作用。方法選取青島市海慈醫療集糰普外科收治的膽道疾病患者100例，按照良、噁性將其分為對照組（良性膽道疾病）和觀察組（膽管癌），每組各50例。取兩組患者膽汁滴于硝痠纖維膜上，通過比較麥胚凝集素（ WGA ）、歐曼陀囉凝集素（ DSA）、小扁豆凝集素（ LCA）、刀豆凝集素（ CONA）試驗暘性率，探討膽汁糖蛋白糖鏈結構變化與良噁性膽道疾病的關繫。結果對照組 WGA、DSA、LCA、CONA 凝集素試驗暘性率分彆為22．0％、14．0％、2．0％、76.0％，觀察組分彆為76．0％、66．0％、76．0％、82．0％。兩組CONA凝集素試驗暘性率差異無統計學意義（P＞0．05），觀察組WGA、LCA、DSA凝集試驗暘性率均明顯高于對照組，差異均有統計學意義（χ2＝29．17、28．17、57．55，均P＜0．05）。結論膽汁糖蛋白糖鏈結構變化與膽道疾病良、噁性密切相關，可以通過WGA、LCA、DSA凝集試驗暘性率判斷膽管疾病良、噁性，值得在臨床上廣汎推廣。
목적：탐토담즙당단백당련결구변화대우감별량악성담도질병적작용。방법선취청도시해자의료집단보외과수치적담도질병환자100례，안조량、악성장기분위대조조（량성담도질병）화관찰조（담관암），매조각50례。취량조환자담즙적우초산섬유막상，통과비교맥배응집소（ WGA ）、구만타라응집소（ DSA）、소편두응집소（ LCA）、도두응집소（ CONA）시험양성솔，탐토담즙당단백당련결구변화여량악성담도질병적관계。결과대조조 WGA、DSA、LCA、CONA 응집소시험양성솔분별위22．0％、14．0％、2．0％、76.0％，관찰조분별위76．0％、66．0％、76．0％、82．0％。량조CONA응집소시험양성솔차이무통계학의의（P＞0．05），관찰조WGA、LCA、DSA응집시험양성솔균명현고우대조조，차이균유통계학의의（χ2＝29．17、28．17、57．55，균P＜0．05）。결론담즙당단백당련결구변화여담도질병량、악성밀절상관，가이통과WGA、LCA、DSA응집시험양성솔판단담관질병량、악성，치득재림상상엄범추엄。
Objective To investigate the effects of changes in protein bile sugar chain structure for differen-tiating benign and malignant biliary tract disease .Methods 100 patients with biliary tract diseases who were treated in Department of General Surgery ,Qingdao Haici Medical Group were selected in this study .According to benign or malignant biliary disease ,the patients were divided into control group ( benign biliary tract disease ) and observation group ( cholangiocarcinoma ) ,50 cases in each group .Two groups of patients with bile drops on the nitrocellulose mem-brane,through the comparison of wheat germ agglutinin (WGA),Datura stramonium agglutinin (DSA),lentil lectin (LCA),concanavalin A(CONA) positive rate of test,to explore the relationship between sugar chain of glycoprotein in bile and bile duct benign and malignant diseases .Results In control group,the positive rates of WGA,DSA,LCA, CONA agglutinin test were 22.0%,14.0%,2.0%,76.0%,those in the observation group were 76.0%,66.0%, 76.0%,82.0% respectively.No statistically significant difference between the two groups of CONA agglutinin test positive rate was observed(P>0.05).The WGA,LCA,DSA agglutination test positive rates in observation group were significantly higher than those in the control group ,the differences were statistically significant (χ2 =29.17,28.17, 57.55,all P<0.05).Conclusion Changes in bile glycoprotein chain structure are closely related to benign and ma -lignant biliary tract disease ,positive rates of WGA ,LCA,DSA agglutination test can be used to determine the benign and malignant biliary disease ,it should be popularized in clinical practice .
