CAJ | 학술논문

目的：将骨髓间充质干细胞的移植引入大鼠皮肤损伤的治疗中，观察大鼠骨髓间充质干细胞注射治疗皮肤损伤的有效性和安全性。方法体外完成大鼠骨髓间充质干细胞的分离、培养、纯化、扩增和传代；大鼠的分组并建立皮肤损伤模型；骨髓间充质干细胞局部注射；Western blot检测大鼠创面角质化细胞生长因子（ KGF）蛋白的表达；建模后连续创面组织病理学观察。结果实验组建模后3 d、7 d、14 d创面愈合率分别为（21．95±8．11）％、（56．25±4．96）％、（79．85±5．44）％，明显优于同期对照组的（12.11±4．87）％、（30．24±1．53）％、（58．51±6．52）％（ t＝4．693、3．853、2．559，均P＜0．05）。建模后14 d，实验组与对照组KGF蛋白的Western Blot条带显示结果显示实验组创面组织中的KGF蛋白表达较高；观察HE染色后的皮肤组织切片发现，建模后14 d实验组小鼠创面愈合情况优于对照组。结论将骨髓间充质干细胞的移植治疗引入大鼠皮肤损伤的治疗，可以促进大鼠皮肤损伤的愈合。
목적：장골수간충질간세포적이식인입대서피부손상적치료중，관찰대서골수간충질간세포주사치료피부손상적유효성화안전성。방법체외완성대서골수간충질간세포적분리、배양、순화、확증화전대；대서적분조병건립피부손상모형；골수간충질간세포국부주사；Western blot검측대서창면각질화세포생장인자（ KGF）단백적표체；건모후련속창면조직병이학관찰。결과실험조건모후3 d、7 d、14 d창면유합솔분별위（21．95±8．11）％、（56．25±4．96）％、（79．85±5．44）％，명현우우동기대조조적（12.11±4．87）％、（30．24±1．53）％、（58．51±6．52）％（ t＝4．693、3．853、2．559，균P＜0．05）。건모후14 d，실험조여대조조KGF단백적Western Blot조대현시결과현시실험조창면조직중적KGF단백표체교고；관찰HE염색후적피부조직절편발현，건모후14 d실험조소서창면유합정황우우대조조。결론장골수간충질간세포적이식치료인입대서피부손상적치료，가이촉진대서피부손상적유합。
Objective This experiment used the allogeneic bone marrow mesenchymal stem cells to cure the wound in rats .And to observe the effect and safety of bone marrow mesenchymal stem cells injection for wound heal -ing.Methods The rats with wound were divided into two groups .The rats bone marrow mesenchymal stem cells were isolated,cultured,purified,amplified and passaged.Flow cytometry instrument was used to identify the immune pheno-type of rats bone marrow mesenchymal stem cells .Local injected the rats-bone marrow mesenchymal stem cells in the rats.The difference of wound healing rate between the experimental group and control group were statistically ana -lyzed.Western blot was used to detect the expression of keratinocyte growth factor (KGF) in two groups.Histopatho-logical change of the wound was observed .Results The wound healing rate between the two groups had significantly statistical difference.After 3 days,7 days,14 days,the wound healing rates of the experimental group were (21.95 ± 8.11)%,(56.25 ±4.96)% and (79.85 ±5.44)%,respectively and which were better than those of the control group[(12.11 ±4.87)%,(30.24 ±1.53)%,(58.51 ±6.52)%](t=4.693,3.853,2.559,all P <0.05). According to the grading standards of the healing skin after HE staining ,the experimental group was better than the control group .According to the results of Western blot , the KGF protein expression of the experimental group was higher than the control group .Conclusion The allogeneic bone marrow mesenchymal stem cells may be an effective and safe method for the wound in rats .