中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
22期
56-58
,共3页
离子色谱%合成多肽类药物%醋酸
離子色譜%閤成多肽類藥物%醋痠
리자색보%합성다태류약물%작산
Ion chromatography%Synthetic polypeptide drug%Acetic acid
目的：探索采用离子色谱法测定合成多肽药物中醋酸的含量。方法以DIONEX IonPac AS19(4 mm×250 mm)为色谱柱,淋洗液为3.2 mmol/L的碳酸钠和1.0 mmol/L碳酸氢钠混合溶液,流速为0.8 ml/min,抑制电流为70 mA,电导检测器进行检测。结果醋酸含量在14.2~142.2μg/ml范围内线性相关系数为0.9997,回收率在98.1%~100.7%,RSD为1.0%。结论离子色谱法简便快捷,结果准确,可替代高效液相色谱法用于测定合成多肽药物中醋酸的含量。
目的：探索採用離子色譜法測定閤成多肽藥物中醋痠的含量。方法以DIONEX IonPac AS19(4 mm×250 mm)為色譜柱,淋洗液為3.2 mmol/L的碳痠鈉和1.0 mmol/L碳痠氫鈉混閤溶液,流速為0.8 ml/min,抑製電流為70 mA,電導檢測器進行檢測。結果醋痠含量在14.2~142.2μg/ml範圍內線性相關繫數為0.9997,迴收率在98.1%~100.7%,RSD為1.0%。結論離子色譜法簡便快捷,結果準確,可替代高效液相色譜法用于測定閤成多肽藥物中醋痠的含量。
목적：탐색채용리자색보법측정합성다태약물중작산적함량。방법이DIONEX IonPac AS19(4 mm×250 mm)위색보주,림세액위3.2 mmol/L적탄산납화1.0 mmol/L탄산경납혼합용액,류속위0.8 ml/min,억제전류위70 mA,전도검측기진행검측。결과작산함량재14.2~142.2μg/ml범위내선성상관계수위0.9997,회수솔재98.1%~100.7%,RSD위1.0%。결론리자색보법간편쾌첩,결과준학,가체대고효액상색보법용우측정합성다태약물중작산적함량。
Objective To explore the content of acetic acid in synthetic polypeptide drugs determined by ion chro-matography. Methods Analytical column was DIONEX IonPac AS19 (4 mmí250 mm),the leacheate was a solution of 3.2 mmol/L sodium carbonate mixed with 1.0 mmol/L sodium bicarbonate,flow velocity was 0.8 ml per minute,the curb was 70 mA,and electrical conductivity detector was applied for determination. Results The linearly dependent coeffi-cient was 0.9997 when the content of acetic acid ranged from 14.2 μg/ml to 142.2 μg/ml.The recovery rate was from 98.1% to 100.7% and percentage of relative standard deviation (RSD) was 1.0%. Conclusion Ion chromatography is simple,convenient and fast with accurate result,which can replace high performance liquid chromatography in determin-ing the content of acetic acid from synthetic polypeptide drugs.
