中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
23期
118-121
,共4页
抗DNA酶B%抗链球菌溶血素O%链球菌感染%风湿热%急性肾小球肾炎
抗DNA酶B%抗鏈毬菌溶血素O%鏈毬菌感染%風濕熱%急性腎小毬腎炎
항DNA매B%항련구균용혈소O%련구균감염%풍습열%급성신소구신염
Anti-DNA enzyme B%Anti-streptolysin O%Streptococcus infection%Rheumatic fever%Acute glomerulonephritis
目的：探讨抗DNA酶B对小儿链球菌感染相关疾病的价值。方法收集本院2012年1月~2013年10月诊断为链球菌感染的患儿114例,将其分为急性风湿热组、急性咽炎和扁桃体炎组、急性肾小球肾炎组、风湿性心脏病组,同时选取健康儿童30例为对照组。用微量中和法检测抗DNA酶B,特定蛋白仪免疫散射比浊法检测抗链球菌溶血素O(ASO),并同时检测咽拭子培养、C反应蛋白(CRP)、红细胞沉降率(ESR)、血常规,分别测定各组的阳性率并进行比较。结果急性风湿热组、急性咽炎和扁桃体炎组、急性肾小球肾炎组抗DNA酶B阳性率分别为70.8%、33.3%、83.3%,高于ASO阳性率54.2%、23.3%、60.0%,差异有统计学意义(P<0.05)；两者联合检测阳性率分别为91.7%、53.3%、93.3%,均高于ASO单独检测阳性率,差异有统计学意义(P<0.05)。风湿性心脏病活动期抗DNA酶B阳性率为87.5%,明显高于非活动期抗DNA酶B阳性率21.4%,差异有统计学意义(P<0.05)。结论抗DNA酶B及ASO检测对于小儿链球菌感染病程的不同时期有不同意义,联合检测能明显提高检出阳性率,抗DNA酶B对判断链球菌感染相关疾病活动有一定意义。
目的：探討抗DNA酶B對小兒鏈毬菌感染相關疾病的價值。方法收集本院2012年1月~2013年10月診斷為鏈毬菌感染的患兒114例,將其分為急性風濕熱組、急性嚥炎和扁桃體炎組、急性腎小毬腎炎組、風濕性心髒病組,同時選取健康兒童30例為對照組。用微量中和法檢測抗DNA酶B,特定蛋白儀免疫散射比濁法檢測抗鏈毬菌溶血素O(ASO),併同時檢測嚥拭子培養、C反應蛋白(CRP)、紅細胞沉降率(ESR)、血常規,分彆測定各組的暘性率併進行比較。結果急性風濕熱組、急性嚥炎和扁桃體炎組、急性腎小毬腎炎組抗DNA酶B暘性率分彆為70.8%、33.3%、83.3%,高于ASO暘性率54.2%、23.3%、60.0%,差異有統計學意義(P<0.05)；兩者聯閤檢測暘性率分彆為91.7%、53.3%、93.3%,均高于ASO單獨檢測暘性率,差異有統計學意義(P<0.05)。風濕性心髒病活動期抗DNA酶B暘性率為87.5%,明顯高于非活動期抗DNA酶B暘性率21.4%,差異有統計學意義(P<0.05)。結論抗DNA酶B及ASO檢測對于小兒鏈毬菌感染病程的不同時期有不同意義,聯閤檢測能明顯提高檢齣暘性率,抗DNA酶B對判斷鏈毬菌感染相關疾病活動有一定意義。
목적：탐토항DNA매B대소인련구균감염상관질병적개치。방법수집본원2012년1월~2013년10월진단위련구균감염적환인114례,장기분위급성풍습열조、급성인염화편도체염조、급성신소구신염조、풍습성심장병조,동시선취건강인동30례위대조조。용미량중화법검측항DNA매B,특정단백의면역산사비탁법검측항련구균용혈소O(ASO),병동시검측인식자배양、C반응단백(CRP)、홍세포침강솔(ESR)、혈상규,분별측정각조적양성솔병진행비교。결과급성풍습열조、급성인염화편도체염조、급성신소구신염조항DNA매B양성솔분별위70.8%、33.3%、83.3%,고우ASO양성솔54.2%、23.3%、60.0%,차이유통계학의의(P<0.05)；량자연합검측양성솔분별위91.7%、53.3%、93.3%,균고우ASO단독검측양성솔,차이유통계학의의(P<0.05)。풍습성심장병활동기항DNA매B양성솔위87.5%,명현고우비활동기항DNA매B양성솔21.4%,차이유통계학의의(P<0.05)。결론항DNA매B급ASO검측대우소인련구균감염병정적불동시기유불동의의,연합검측능명현제고검출양성솔,항DNA매B대판단련구균감염상관질병활동유일정의의。
Objective To investigate the value of anti-DNA enzyme B on related diseases in children with Streptococ-cus infection. Methods 114 cases of patients with Streptococcus infection in our hospital from January 2012 to October 2013 were collected,and were divided into acute rheumatic fever group,acute pharyngitis and tonsillitis group,acute glomerulonephritis group,rheumatic heart disease group,at the same time,30 cases of healthy children were selected as control group.Anti-DNA enzyme B was detected by micro-neutralization method,Anti-streptolysin O (ASO) was detect-ed by specific protein analyzer immune nephelometry,and pharyngeal swab culture,C-reactive protein (CRP),erythrocyte sedimentation tate (ESR),blood routine were detected,at last the positive rate of each group were compared. Results Acute rheumatic fever group,acute pharyngitis and tonsillitis group,acute glomerulonephritis group of anti-DNA enzyme B positive rate was 70.8%,33.3%,83.3% respectively,higher than 54.2%,23.3%,60.0% of ASO positive rate,the differ-ence was significant (P<0.05);the positive rate of combined detection was 91.7%,53.3%,93.3%,higher than the positive rate of ASO detection alone,the difference was significant (P<0.05).Active rheumatic heart disease of anti-DNA enzyme B positive detection rate was 87.5%,higher than 21.4% of unactive rheumatic heart disease,the difference was signifi-cant (P<0.05). Conclusion The detection of anti-DNA enzyme B and ASO have different meanings for different stages of children with Streptococcus infection,joint detection of two methods can improve the positive rate,anti-DNA enzyme B has certain significance to the activities related to judge the Streptococcus infection disease.
