CAJ | 학술논문

目的：探讨伴神经内分泌分化(NED)结直肠腺癌中p53、Ki-67的表达及其与预后的关系。方法采用SP法检测NSE、CgA、Syn、CD-57及p53、Ki-67蛋白在65例结直肠腺癌组织中的表达,分为NED阳性组35例和NED阴性组30例,并分析p53、Ki-67表达对预后的影响。结果 NED阳性组中p53、Ki-67阳性表达率均明显高于NED阴性组(P<0.05)。p53、Ki-67的表达与淋巴结转移、组织学分化和DUCK分期密切相关(P<0.05)。NED阴性组的5年生存率明显高于NED阳性组(P<0.05)；NED阳性组中,p53表达阴性者和Ki-67表达阴性者的5年生存率明显高于两者表达阳性者(P<0.05)。 Cox回归分析表明,淋巴结转移、DUCK分期、p53及Ki-67表达是患者5年生存率的独立预后因素(P<0.05)。结论 p53、Ki-67的高表达可能在伴NED结直肠腺癌发生发展过程中起重要作用；两者均为伴NED结直肠腺癌预后的独立危险因素。
목적：탐토반신경내분비분화(NED)결직장선암중p53、Ki-67적표체급기여예후적관계。방법채용SP법검측NSE、CgA、Syn、CD-57급p53、Ki-67단백재65례결직장선암조직중적표체,분위NED양성조35례화NED음성조30례,병분석p53、Ki-67표체대예후적영향。결과 NED양성조중p53、Ki-67양성표체솔균명현고우NED음성조(P<0.05)。p53、Ki-67적표체여림파결전이、조직학분화화DUCK분기밀절상관(P<0.05)。NED음성조적5년생존솔명현고우NED양성조(P<0.05)；NED양성조중,p53표체음성자화Ki-67표체음성자적5년생존솔명현고우량자표체양성자(P<0.05)。 Cox회귀분석표명,림파결전이、DUCK분기、p53급Ki-67표체시환자5년생존솔적독립예후인소(P<0.05)。결론 p53、Ki-67적고표체가능재반NED결직장선암발생발전과정중기중요작용；량자균위반NED결직장선암예후적독립위험인소。
Objective To investigate the expression of p53, Ki-67 in colorectal adenocarcinoma with neuroendocrine differentiation (NED), and to explore the correlation between the expression and its prognosis. Methods The expression of NSE, CgA, Syn, CD-57, p53, Ki-67 in 65 colorectal adenocarcinoma were determined by using immunohistochemi-cal staining. According the neuroendocrine index, 65 cases were divided into two groups,NED positive group(35 cases) and NED negative group (30 cases),and analyzed the influence of erpression of p53, ki-67cm prognosis. Results The positive expression rates of p53, Ki-67 in NED positive group were higher than those in NED negative group (P<0.05). The expression of p53,Ki-67 in NED positive group were significantly associated with lymph node metastasis, differen-tiation and DUCK stages (P<0.05). The 5-year survival rate of NED negative group was obviously higher than that of NED positive group (P<0.05);In NED positive group, the 5-year survival rate of p53 negative and Ki-67 negative ex-pression were significantly higher than that of p53 positive and Ki-67 positive expression (P<0.05). Using Cox regres-sion, lymph node metastasis, differentiation, DUCK stages and the expression of p53,Ki-67 were strong independent prognostic factors for five years of disease-free survival rate of colorectal adenocarcinoma with NED patients (P<0.05). Conclusion The high expression of p53, Ki-67 may be associated with colorectal adenocarcinoma with NED and play an important role in malignant proliferation. p53,Ki-67 are valuable prognostic factors for colorectal adenocarcinoma with NED patients.