CAJ | 학술논문

目的：探讨快速培养法在诊断肺炎支原体导致的呼吸道感染中的作用。方法对2011年12月～2013年12月来院就诊的疑似肺炎支原体感染患者200例进行研究，留取咽拭子和血清，分别进行快速培养法、荧光定量PCR法和血清抗体法检验，并就快速培养法与其余两种不同的检测方法的结果进行对比分析。结果快速培养法、荧光定量PCR法和血清抗体检验法检测阳性率分别为30.0%（60/200）、29.0%（58/200）和16.5%（33/200），快速培养法阳性率与荧光定量PCR法检测阳性率相比较，差异无统计学意义（P＞0.05）；而快速培养法检测阳性率与血清抗体法检测阳性率相比较，快速培养法检测阳性率显著高于血清抗体检测法，且差异具有统计学意义（P＜0.05）。结论快速培养法检测肺炎支原体的敏感性和灵敏性可与荧光定量PCR法检测结果相媲美，但装备技术要求和检测成本明显低于荧光定量PCR法，同时敏感性和灵敏性显著高于血清抗体检测法。快速培养法检测肺炎支原体不仅能有效满足临床需要，同时兼具有成本低的特点，因此这种方法具有较高的性价比，尤其适用于基层医院。
목적：탐토쾌속배양법재진단폐염지원체도치적호흡도감염중적작용。방법대2011년12월～2013년12월래원취진적의사폐염지원체감염환자200례진행연구，류취인식자화혈청，분별진행쾌속배양법、형광정량PCR법화혈청항체법검험，병취쾌속배양법여기여량충불동적검측방법적결과진행대비분석。결과쾌속배양법、형광정량PCR법화혈청항체검험법검측양성솔분별위30.0%（60/200）、29.0%（58/200）화16.5%（33/200），쾌속배양법양성솔여형광정량PCR법검측양성솔상비교，차이무통계학의의（P＞0.05）；이쾌속배양법검측양성솔여혈청항체법검측양성솔상비교，쾌속배양법검측양성솔현저고우혈청항체검측법，차차이구유통계학의의（P＜0.05）。결론쾌속배양법검측폐염지원체적민감성화령민성가여형광정량PCR법검측결과상비미，단장비기술요구화검측성본명현저우형광정량PCR법，동시민감성화령민성현저고우혈청항체검측법。쾌속배양법검측폐염지원체불부능유효만족림상수요，동시겸구유성본저적특점，인차저충방법구유교고적성개비，우기괄용우기층의원。
Objective To investigate the early diagnosis of pulmonary mycoplasma pneumoniae infection. Methods 200 cases adults with respiratory infection was tested by sputum, pharynx swab culture and fluorogenic quantitative PCR. The diagnosis results were compared in the same cases. Results The positive rate of the pharynx swab culture and fluorogenic quantitative PCR was 30.0%(60/200),29.0%(58/200)respectively. There was no significant difference between the two methods(P > 0.05). However,the positive rate of the sputum was lowest in the three methods. There was a significant different in the comparison of the data(P<0.05). Conclusion It would get a satisfactory diagnosis effect to use mycoplasma pneumoniae rapid liquid culture in respiratory disease.It has the same effect with the PCR, but lower cost and the need of equipment requirement.