CAJ | 학술논문

水稻细菌性条斑病菌(Xanthomonas oryzae pv．oryzicola ,Xoc)是一种重要植物病原菌,其引发的水稻细菌性条斑病导致水稻严重减产.Xoc 一个假定的 udg H 基因的突变,导致 Xoc 产胞外多糖和致病力的严重减弱,并使胞外淀粉酶和纤维素酶的酶活也有一定的降低,互补菌株能恢复到野生型水平,但致病力仅能恢复到80％,而高表达菌株与野生型相比胞外多糖产量增加,但致病力降低.本研究为进一步研究该基因在水稻条斑病菌中胞外多糖代谢途径的作用奠定了基础,并为利用基因工程手段改造高产黄原胶菌种提供了一种可行的策略.
수도세균성조반병균(Xanthomonas oryzae pv．oryzicola ,Xoc)시일충중요식물병원균,기인발적수도세균성조반병도치수도엄중감산.Xoc 일개가정적 udg H 기인적돌변,도치 Xoc 산포외다당화치병력적엄중감약,병사포외정분매화섬유소매적매활야유일정적강저,호보균주능회복도야생형수평,단치병력부능회복도80％,이고표체균주여야생형상비포외다당산량증가,단치병력강저.본연구위진일보연구해기인재수도조반병균중포외다당대사도경적작용전정료기출,병위이용기인공정수단개조고산황원효균충제공료일충가행적책략.
Xanthomonas oryzae pv.oryzicola (Xoc)is an important plant pathogen,which caused rice bacterial leaf blight and seriously decreased the production of rice.A mutation in putative udg H gene of Xoc leads to serious weakening on extracellular polysaccharide production and virulence,and lower ex-tracellular amylase and cellulase activity.The phenotype of complementary strain can be restored to wild-type levels,while virulence only by 80%,and the extracellular polysaccharide production of high expression strain is increased,while the virulence is decreased.The research laid solid foundation for further study the role of the gene in extracellular polysaccharide metabolic pathways of Xoc,and provid-ed a feasible method for constructing high-yielding strains of xanthan gum useing genetic engineering.