CAJ | 학술논문

目的：运用基因芯片技术分析沈阳地区HBV基因型分布特征及基因耐药变异情况。方法随机选择2012年8月至2013年7月中国人民解放军二0二医院乙型肝炎住院患者血清样本90例，应用巢式聚合酶链反应与反向点杂交相结合的基因芯片技术对样本血清中 HBV基因型及常见3类抗病毒药物耐药相关的五个位点进行检测，并进行数据分析。结果90例样本中HBV基因型包括：B型6．7％（6/90）、C型86．6％（78/90）、B和C混合型6．7％（6/90）。HBV-C基因型患者相对B型及B、C混合型患者血清中的HBV DNA平均水平偏高。受检样本中有4例发生耐药基因变异，均出现于C基因型患者，其中3例为180M＋204V突变型，1例为180M＋204I突变型。结论沈阳地区HBV基因型分布以C型为主，B型及B、C混合基因型较少。HBV-C基因型患者 HBV DNA水平相对偏高，更容易发生对拉米夫定耐药，且以180M混合型突变最易发生。
목적：운용기인심편기술분석침양지구HBV기인형분포특정급기인내약변이정황。방법수궤선택2012년8월지2013년7월중국인민해방군이0이의원을형간염주원환자혈청양본90례，응용소식취합매련반응여반향점잡교상결합적기인심편기술대양본혈청중 HBV기인형급상견3류항병독약물내약상관적오개위점진행검측，병진행수거분석。결과90례양본중HBV기인형포괄：B형6．7％（6/90）、C형86．6％（78/90）、B화C혼합형6．7％（6/90）。HBV-C기인형환자상대B형급B、C혼합형환자혈청중적HBV DNA평균수평편고。수검양본중유4례발생내약기인변이，균출현우C기인형환자，기중3례위180M＋204V돌변형，1례위180M＋204I돌변형。결론침양지구HBV기인형분포이C형위주，B형급B、C혼합기인형교소。HBV-C기인형환자 HBV DNA수평상대편고，경용역발생대랍미부정내약，차이180M혼합형돌변최역발생。
Objective To investigate the distribution characteristics of hepatitis B virus (HBV) genotypes and the variation situ-ation of drug resistance gene in Shenyang area by the gene chip technology .Methods Serum samples were randomly collected from 90 inpatients of hepatitis B in 202 Hospital of PLA from August 2012 to July 2013 .The HBV genotypes and drug resistance associ-ated 5 sites in common 3 types of antiviral drug were detected by the gene chip technology of nested PCR (NPCR) combined with reverse dot blot(RDB) .Results Among 90 samples ,HBV genotypes included type B 6 .7% (6/90) ,type C 86 .6% (78/90) ,mixed type B and C 6 .7% (6/90) .The serum HBV DNA level in the patients with HBV-C genotype were significantly higher than that with genotype HBV-B and mixed genotype B and C .There were 4 cases of drug resistance gene mutation in the detected samples ,3 cases of mutation were placed in 180M+204V ,1 case of mutation was placed in 180M+204I .All the drug resistance mutations ap-peared in the patients with HBV-C genotype .Conclusion The main HBV genotype is the genotype C in Shenyang area .Genotype B and genotype mixed B and C are unusual .The HBV DNA level in the patients with the genotype C is relatively high ,which is more likely to happen resistance to lamivudine .Moreover the mixed mutation in 180M is more likely to happen .