CAJ | 학술논문

目的：分析该院女性人乳头瘤病毒（H PV ）感染情况及基因亚型的分布情况，为该院临床防治宫颈感染、研制适合该院预防性HPV疫苗提供理论依据。方法采用 PCR-反向点杂交法对该院625例妇科门诊就诊以及住院患者宫颈刷片进行HPV基因型检查。结果625例患者中HPV感染210例，感染总阳性率33．60％，单一亚型感染152例，占72．38％，大于或等于两种亚型混合感染者58例，占27．62％；感染率较高的亚型从高到低依次为HPV58（7．52％）、HPV16（6．72％）、HPV6（5．76％）、HPV43（4．80％）、HPV11（4．64％）、HPV35（3．36％）、HPV56（2．56％）、HPV18（2．40％）、HPV52（2．40％）和 HPV68（2．40％）型。结论该院妇科门诊及住院患者的 HPV感染率较高，亚型分布较广泛且以高危 HPV感染为主，最常见的亚型 HPV58，多重H PV感染率较高。
목적：분석해원녀성인유두류병독（H PV ）감염정황급기인아형적분포정황，위해원림상방치궁경감염、연제괄합해원예방성HPV역묘제공이론의거。방법채용 PCR-반향점잡교법대해원625례부과문진취진이급주원환자궁경쇄편진행HPV기인형검사。결과625례환자중HPV감염210례，감염총양성솔33．60％，단일아형감염152례，점72．38％，대우혹등우량충아형혼합감염자58례，점27．62％；감염솔교고적아형종고도저의차위HPV58（7．52％）、HPV16（6．72％）、HPV6（5．76％）、HPV43（4．80％）、HPV11（4．64％）、HPV35（3．36％）、HPV56（2．56％）、HPV18（2．40％）、HPV52（2．40％）화 HPV68（2．40％）형。결론해원부과문진급주원환자적 HPV감염솔교고，아형분포교엄범차이고위 HPV감염위주，최상견적아형 HPV58，다중H PV감염솔교고。
Objective To investigate the female infection situation of human papilloma virus (HPV) and the distribution charac-teristics of HPV gene subtypes in the hospital ,so as to provide a theoretical basis for clinical prevention and treatment of HPV cer-vical infection and the study for developing the preventive HPV vaccine suitable for this hospital .Methods The PCR-reverse dot blot hybridization (PCR-RDB) was adopted to check the genotypes of HPV on the cervical samples in 625 gynecologic outpatients and inpatients .Results Among 625 cases ,210 cases were infected by HPV with the total HPV infection rate of 33 .6% .The single subtype infection was in 152 cases ,accounting for 72 .38% ,the mixed infection by more than or equal to 2 kinds of subtype was in 58 cases ,accounting for 27 .62% .The subtypes with the higher infection rate from high to low were HPV 58(7 .52% ) ,HPV16 (6 .72% ) ,HPV6(5 .76% ) ,HPV43(4 .80% ) ,HPV11(4 .64% ) ,HPV35(3 .36% ) ,HPV56(2 .56% ) ,HPV18(2 .40% ) ,HPV52 (2 .40% ) and HPV68(2 .40% ) .Conclusion The HPV infection rate in the gynecologic outpatients and inpatients in the hospital is high ,the subtype distribution is wider and is dominated by the high risk HPV infection .The most common subtype is HPV58 and multiple HPV infection rate is high .