CAJ | 학술논문

目的：川东北地区健康人群抗突变型瓜氨酸波形蛋白抗体（anti-MCV）的参考范围的建立。方法收集健康人群血清141份，采用Alegria酶联免疫分析仪及仪器配套的anti-MCV检测试剂盒检测血清anti-MCV浓度。结果 anti-MCV检测系统的批内和天间精密度以及功能灵敏度均符合实验室要求。健康人群女性组血清anti-MCV浓度为12．10（6．70～41．30）U/mL ，与男性组anti-MCV浓度为11．10（6．00～18．30）U/mL比较，差异无统计学意义（Z＝-0．991，P＝0．322）；＜50岁年龄组和大于或等于50岁年龄组血清 anti-MCV 检测结果比较差异无统计学意义（Z＝-0．588，P＝0．558）。血清 anti-MCV 参考范围为0．00～18．14 U/mL。结论 anti-MCV参考范围与性别和年龄均无关系，建立适合该地区健康人群的anti-MCV参考范围具有非常重要的临床应用价值。
목적：천동북지구건강인군항돌변형과안산파형단백항체（anti-MCV）적삼고범위적건립。방법수집건강인군혈청141빈，채용Alegria매련면역분석의급의기배투적anti-MCV검측시제합검측혈청anti-MCV농도。결과 anti-MCV검측계통적비내화천간정밀도이급공능령민도균부합실험실요구。건강인군녀성조혈청anti-MCV농도위12．10（6．70～41．30）U/mL ，여남성조anti-MCV농도위11．10（6．00～18．30）U/mL비교，차이무통계학의의（Z＝-0．991，P＝0．322）；＜50세년령조화대우혹등우50세년령조혈청 anti-MCV 검측결과비교차이무통계학의의（Z＝-0．588，P＝0．558）。혈청 anti-MCV 삼고범위위0．00～18．14 U/mL。결론 anti-MCV삼고범위여성별화년령균무관계，건립괄합해지구건강인군적anti-MCV삼고범위구유비상중요적림상응용개치。
Objective To establish the reference range of anti-mutated citrullinated vimentin antibody (anti-MCV ) among healthy individuals in Northeast area of Sichuan .Methods The Alegria enzyme-linked immunosorbent assay system and the matched anti-MCH reagent test kit were adopted to detect the serum anti-MCV concentration in 141 serum samples collected from healthy individuals .Results The intra-batch and inter-batch precisions and the functional sensitivity of the Alegria enzyme-linked immunosorbent assay system all met the laboratory requirements .The concentration of serum anti-MCV was 12 .10(6 .70-41 .30) U/mL in the healthy female group and 11 .10(6 .00-18 .30)U/mL in the healthy male group ,there was no statistical difference be-tween these two groups(Z= -0 .991 ,P=0 .322);the detection results of serum anti-MCV in the age groups of <50 years old and ≥50 years old had no statistical differences (Z= -0 .588 ,P= 0 .558) .The reference range of anti-MCV in the investigated healthy individuals was 0 .00-18 .14 U/mL .Conclusion The reference range of anti-MCV has no correlation with gender and age . Establishing the reference range of anti-MCV suitable for the healthy population in the local area has very important clinical applica-tion value .