CAJ | 학술논문

目的：探讨可溶性B7-H4（sB7-H4）在类风湿性关节炎（RA）中的诊断价值。方法应用酶联免疫吸附试验（ELISA）检测67例RA患者，72例骨性关节炎（OA）与75例健康人血清sB7-H4、抗环瓜氨酸肽（CCP）抗体、葡萄糖6-磷酸异构酶（GPI）水平，免疫比浊法测定类风湿因子（RF）。比较分析sB7-H4在RA诊断中的应用价值。结果 RA患者血清sB7-H4水平明显高于对照组和OA组，差异有统计学意义（P＜0．01）；sB7-H4在RA诊断中的灵敏度高于 RF、抗CCP抗体、GPI ，特异性低于抗CCP抗体及GPI ；sB7-H4与RF、抗CCP抗体有良好的相关性；联合检测sB7-H4、RF、抗CCP抗体、GPI可提高RA的诊断率。结论sB7-H4在RA诊断中具有良好的临床应用前景。
목적：탐토가용성B7-H4（sB7-H4）재류풍습성관절염（RA）중적진단개치。방법응용매련면역흡부시험（ELISA）검측67례RA환자，72례골성관절염（OA）여75례건강인혈청sB7-H4、항배과안산태（CCP）항체、포도당6-린산이구매（GPI）수평，면역비탁법측정류풍습인자（RF）。비교분석sB7-H4재RA진단중적응용개치。결과 RA환자혈청sB7-H4수평명현고우대조조화OA조，차이유통계학의의（P＜0．01）；sB7-H4재RA진단중적령민도고우 RF、항CCP항체、GPI ，특이성저우항CCP항체급GPI ；sB7-H4여RF、항CCP항체유량호적상관성；연합검측sB7-H4、RF、항CCP항체、GPI가제고RA적진단솔。결론sB7-H4재RA진단중구유량호적림상응용전경。
Objective To investigate the diagnosis value of soluble B7-H4(sB7-H4) in rheumatoid arthritis (RA) .Methods By using the ELISA sandwich method ,the serum levels of soluble B7-H4 ,anti-cyclic citrullinated peptide(anti-CCP) antibody and glu-cose 6-phosphate isomerase(GPI) were detected in 67 cases of RA ,72 cases of osteoarthritis(OA) and 75 cases of healthy people . The immunoturbidimetric method was adopted to determine the serum rheumatoid factor (RF) .Then the diagnostic value of sB7-H4 in RA was compared and analyzed .Results The serum level of soluble B7-H4 in the RA group was significantly higher than that in the control group and the OA group with statistically significant difference between them (P<0 .01) .The sensitivity of sB7-H4 in diagnosing RA was higher than that of RF ,anti-CCP antibody and GPI ,but the specificity was lower than that of anti-CCP antibody and GPI .There was better correlation between sB7-H4 with RF and anti-CCP antibody .The combination detecting of sB7-H4 ,RF , anti-CCP antibody and GPI could increase the diagnostic rate of RA .Conclusion B7-H4 has better clinical application prospects in diagnosing RA .