CAJ | 학술논문

目的：应用NCCLS EP7-A2文件对D-3-羟丁酸（D3H）检测试剂盒抗干扰性能进行评价，并对其抗干扰性能进行改进。方法根据NCCLS EP7-A2文件，对D3H检测试剂盒进行干扰评价试验，并通过添加维生素C氧化酶对其抗维生素C干扰性能进行改进。结果342 mol/L游离胆红素、342 mol/L结合胆红素、1450 FTU乳糜、5 g/L血红蛋白对低、高浓度D3H测定无干扰，0．03 g/L维生素C对低、高浓度D3 H测定有干扰。剂量效应实验结果显示，维生素C对D3 H测定可产生线性正干扰。添加2500 U/L维生素C氧化酶可对抗维生素C对检测的干扰。结论维生素C会干扰D3 H的检测，试剂中添加维生素C氧化酶可对抗其干扰。
목적：응용NCCLS EP7-A2문건대D-3-간정산（D3H）검측시제합항간우성능진행평개，병대기항간우성능진행개진。방법근거NCCLS EP7-A2문건，대D3H검측시제합진행간우평개시험，병통과첨가유생소C양화매대기항유생소C간우성능진행개진。결과342 mol/L유리담홍소、342 mol/L결합담홍소、1450 FTU유미、5 g/L혈홍단백대저、고농도D3H측정무간우，0．03 g/L유생소C대저、고농도D3 H측정유간우。제량효응실험결과현시，유생소C대D3 H측정가산생선성정간우。첨가2500 U/L유생소C양화매가대항유생소C대검측적간우。결론유생소C회간우D3 H적검측，시제중첨가유생소C양화매가대항기간우。
Objective To evaluate the anti-interference performance of D-3-hydroxybutyrate (D3H ) reagent kit by using the NCCLS document EP7-A2 and to improve its anti-interference performance .Methods The anti-interference evaluation test of D3H kit was performed according to NCCLS document EP7-A2 and its anti-interference performance was improved by adding vitamin C oxidase .Results 342 mol/L free bilirubin ,342 mol/L conjugated bilirubin ,1 450 FTU chyle and 5 g/L hematoglobin had no inter-ference on the high concentration D3H detection ,0 .03 g/L vitamin C interfered with the low and high concentration D3H detection . The dosage-effect experiment results showed that vitamin C produced the linear positive interference effect on D 3H detection . Adding 2 500 U/L of vitamin C oxidase could antagonize the interference of vitamin C on detection .Conclusion Vitamin C can in-terfere with the D3H detection and adding vitamin C oxidase in reagent can antagonize its interference .