国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
15期
1974-1975
,共2页
杨燕%李炜%李晓鹤%罗莎莎%李小勇
楊燕%李煒%李曉鶴%囉莎莎%李小勇
양연%리위%리효학%라사사%리소용
补体系统%巨噬细胞%结核病
補體繫統%巨噬細胞%結覈病
보체계통%거서세포%결핵병
complement system%macrophages%tuberculosis
目的：探讨C1q在肺结核患者中的表达及其对巨噬细胞抗结核作用的机制。方法选择活动性肺结核、结核性胸膜炎、结核杆菌潜伏感染者、健康对照者各30例，于入院次日或健康体检时抽取外周静脉血10～20 m L。对有胸腔积液者抽取胸腔积液50 mL ，对行手术治疗的患者取肺组织标本1 cm ×1 cm ×1 cm。采用实时荧光定量PCR（RT-PCR）检测各组外周血巨噬细胞中C1q表达水平，采用免疫组化染色检测肺组织内巨噬细胞C1q表达水平。结果活动性肺结核组、结核性胸膜炎组患者外周血C1qA的表达水平明显高于健康对照组及潜伏感染组（ P＜0．05）。结核性胸膜炎组患者胸腔积液C1qA的表达水平高于活动性肺结核组（ P＜0．05），且两组患者胸腔积液中C1q的表达水平均高于其在外周血中的表达（ P＜0．05）。活动性肺结核组及结核性胸膜炎组巨噬细胞胞浆中C1q均呈强表达，在多核巨噬细胞中C1q的表达较弱。结论结核病患者外周血C1q表达增高，病变局部的免疫反应可能更为明显。C1q在结核巨噬细胞中强表达，可能与巨噬细胞分化和表型有相关性。
目的：探討C1q在肺結覈患者中的錶達及其對巨噬細胞抗結覈作用的機製。方法選擇活動性肺結覈、結覈性胸膜炎、結覈桿菌潛伏感染者、健康對照者各30例，于入院次日或健康體檢時抽取外週靜脈血10～20 m L。對有胸腔積液者抽取胸腔積液50 mL ，對行手術治療的患者取肺組織標本1 cm ×1 cm ×1 cm。採用實時熒光定量PCR（RT-PCR）檢測各組外週血巨噬細胞中C1q錶達水平，採用免疫組化染色檢測肺組織內巨噬細胞C1q錶達水平。結果活動性肺結覈組、結覈性胸膜炎組患者外週血C1qA的錶達水平明顯高于健康對照組及潛伏感染組（ P＜0．05）。結覈性胸膜炎組患者胸腔積液C1qA的錶達水平高于活動性肺結覈組（ P＜0．05），且兩組患者胸腔積液中C1q的錶達水平均高于其在外週血中的錶達（ P＜0．05）。活動性肺結覈組及結覈性胸膜炎組巨噬細胞胞漿中C1q均呈彊錶達，在多覈巨噬細胞中C1q的錶達較弱。結論結覈病患者外週血C1q錶達增高，病變跼部的免疫反應可能更為明顯。C1q在結覈巨噬細胞中彊錶達，可能與巨噬細胞分化和錶型有相關性。
목적：탐토C1q재폐결핵환자중적표체급기대거서세포항결핵작용적궤제。방법선택활동성폐결핵、결핵성흉막염、결핵간균잠복감염자、건강대조자각30례，우입원차일혹건강체검시추취외주정맥혈10～20 m L。대유흉강적액자추취흉강적액50 mL ，대행수술치료적환자취폐조직표본1 cm ×1 cm ×1 cm。채용실시형광정량PCR（RT-PCR）검측각조외주혈거서세포중C1q표체수평，채용면역조화염색검측폐조직내거서세포C1q표체수평。결과활동성폐결핵조、결핵성흉막염조환자외주혈C1qA적표체수평명현고우건강대조조급잠복감염조（ P＜0．05）。결핵성흉막염조환자흉강적액C1qA적표체수평고우활동성폐결핵조（ P＜0．05），차량조환자흉강적액중C1q적표체수평균고우기재외주혈중적표체（ P＜0．05）。활동성폐결핵조급결핵성흉막염조거서세포포장중C1q균정강표체，재다핵거서세포중C1q적표체교약。결론결핵병환자외주혈C1q표체증고，병변국부적면역반응가능경위명현。C1q재결핵거서세포중강표체，가능여거서세포분화화표형유상관성。
Objective To investigate the C1q expression in the patients with pulmonary tuberculosis (TB) and its mechanism on the anti-TB role of macrophages .Methods Each 30 cases of active pulmonary TB ,tuberculous pleurisy ,latent Mycobacterium tu-berculosis infection and healthy controls were selected .Peripheral venous blood 10-20 mL was collected on the next day of admis-sion or on the time for physical examination .Pleural fluid 50 mL was extracted in the patients with hydrothorax and the lung tissue specimens 1 cm × 1 cm × 1 cm was taken in the operative patients .The real-time quantitative PCR(RT-PCR) was adopted to detect the express C1q in peripheral macrophages and the C1q expressing in macrophages of the lung tissue was detected by immunohisto-chemical staining .Results The peripheral blood C1qA expression level in the active pulmonary TB group and the tuberculous pleu-risy group was significantly higher than that in the healthy control group and the latent TB infection group (P<0 .05) .The C1qA expression level in hydrothorax in the tuberculous pleurisy group was higher than that in the active pulmonary TB group (P<0 .05) ,and the C1q expression in hydrothorax in these two groups was higher than that in peripheral blood (P<0 .05) .C1q was strongly expressed in macrophage cytoplasma in the active pulmonary TB group and the TB pleurisy group ,but weakly expressed in the multinuclear macrophages .Conclusion The peripheral blood C1qA expression level in the active pulmonary TB group and the tuberculous pleurisy group was significantly higher than that in the healthy control group and the latent TB infection group (P<0 .05) .The C1qA expression level in hydrothorax in the tuberculous pleurisy group was higher than that in the active pulmonary TB group (P<0 .05) ,and the C1q expression in hydrothorax in these two groups was higher than that in peripheral blood (P<0 .05) . C1q was strongly expressed in macrophage cytoplasma in the active pulmonary TB group and the TB pleurisy group ,but weakly ex-pressed in the multinuclear macrophages .
