CAJ | 학술논문

目的：研究冠心病患者脂联素基因多态性与冠心病患病风险的相关性。方法：256例研究对象中冠心病组126例，对照组130例。采用聚合酶链反应-限制性片段长度多态性法对所有患者的脂联素基因-11，377C ＞ G 位点核苷酸多态性进行基因型分析，采用病例对照方法研究脂联素基因多态性与冠心病风险性的关系。结果：（1）脂联素基因-11，377C ＞ G 位点存在基因多态性，测得基因型为 GG、CG 及CC 型；（2）脂联素基因-11，377C ＞ G 多态性在冠心病组与对照组间的分布存在差异，与对照组比，冠心病组中 G 等位基因的频率有显著升高（P ＜0．05），C 等位基因的频率在冠心病组有显著的降低（P ＜0．05）；（3）脂联素基因-11，377C ＞ G 在急性冠脉综合征组和稳定型心绞痛组间分布无统计学差异（P ＞0．05）；（4）在冠心病组人群中，G 等位基因患者冠心病患病风险明显提高（P ＜0．05）。结论：在冠心病人群脂联素基因-11，377C ＞ G 多态性与冠心病的危险性有关， G 等位基因频率提高可能提示冠心病的患病风险升高。
목적：연구관심병환자지련소기인다태성여관심병환병풍험적상관성。방법：256례연구대상중관심병조126례，대조조130례。채용취합매련반응-한제성편단장도다태성법대소유환자적지련소기인-11，377C ＞ G 위점핵감산다태성진행기인형분석，채용병례대조방법연구지련소기인다태성여관심병풍험성적관계。결과：（1）지련소기인-11，377C ＞ G 위점존재기인다태성，측득기인형위 GG、CG 급CC 형；（2）지련소기인-11，377C ＞ G 다태성재관심병조여대조조간적분포존재차이，여대조조비，관심병조중 G 등위기인적빈솔유현저승고（P ＜0．05），C 등위기인적빈솔재관심병조유현저적강저（P ＜0．05）；（3）지련소기인-11，377C ＞ G 재급성관맥종합정조화은정형심교통조간분포무통계학차이（P ＞0．05）；（4）재관심병조인군중，G 등위기인환자관심병환병풍험명현제고（P ＜0．05）。결론：재관심병인군지련소기인-11，377C ＞ G 다태성여관심병적위험성유관， G 등위기인빈솔제고가능제시관심병적환병풍험승고。
Objective To investigate the relationship between the polymorphism of adiponectin -11 ,377C> G gene and the risk of coronary heart disease(CHD). Methods A total of 126 CHD patients and 130 healthy controls were enrolled and the frequency of each genotypes and allele gene of adiponectin -11 ,377C > G were detected by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Results (1) The adiponectin gene -11,377C > G sites existed gene polymorphism and the three genotypes were GG, CG and CC. (2) There was statistical difference between CHD group and control group; The G allele frequency of CHD group was significantly higher than that in control group (P < 0.05); The frequency of the C allele gene in CHD group was significantly decreased (P < 0.05). (3) There was no statistical difference of frequency distribution of each genotype and allele gene of adiponectin -11,377C > G between acute coronary syndrome (ACS) group and stable angina group . ( 4 ) The risk of CHD were increased in CHD patients with G allele gene of adiponectin-11,377C > G (P < 0.05). Conclusions The polymorphism of adiponectin -11,377C > G is associated with the increased risk of CHD. The increased G allele gene frequency may represent the increased risk of CHD.