CAJ | 학술논문

目的：研究重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）联合纳米银对深Ⅱ°烫伤治疗作用的影响。方法：Wistar大鼠建立深Ⅱ°烫伤模型，分为4组，A组（30只）：凡士林纱布覆盖，B组（30只）：纳米银覆盖，C 组（30只）：rhGM-CSF涂抹，D 组（30只）：rhGM-CSF＋纳米银覆盖，伤后第1、4、7、10、14、21天，观察创面愈合，计算愈合率，酶联免疫吸附法测定血清中血管内皮生长因子（VEGF）和表皮生长因子（EGF）水平。结果：第10天各组开始愈合，愈合过程A组炎症反应明显，B、C组适中，D 组适度；创面愈合率，第10、14、21天组间差异有统计学意义（P＜0．05）；VEGF 水平，A 组第21天达峰值为（25．76±1．46）pg/mL，B、C、D 组第14天达峰值[（29．73±1．58），（38．91±2．38），（43．54±1．28）pg/mL]，第4、7、10、14、21天组间差异有统计学意义（P＜0．05）；EGF水平，各组均在第21天达峰值[（0．72±0．14），（0．93±0．13），（1．18±0．16），（1．50±0．15）ng/mL]，第7、10、14、21天组间差异有统计学意义（P＜0．05）。结论：rhGM-CSF 联合纳米银，促进深Ⅱ°烫伤创面愈合，并且优于rhGM-CSF、纳米银单独应用。
목적：연구중조인립세포거서세포집락자격인자（rhGM-CSF）연합납미은대심Ⅱ°탕상치료작용적영향。방법：Wistar대서건립심Ⅱ°탕상모형，분위4조，A조（30지）：범사림사포복개，B조（30지）：납미은복개，C 조（30지）：rhGM-CSF도말，D 조（30지）：rhGM-CSF＋납미은복개，상후제1、4、7、10、14、21천，관찰창면유합，계산유합솔，매련면역흡부법측정혈청중혈관내피생장인자（VEGF）화표피생장인자（EGF）수평。결과：제10천각조개시유합，유합과정A조염증반응명현，B、C조괄중，D 조괄도；창면유합솔，제10、14、21천조간차이유통계학의의（P＜0．05）；VEGF 수평，A 조제21천체봉치위（25．76±1．46）pg/mL，B、C、D 조제14천체봉치[（29．73±1．58），（38．91±2．38），（43．54±1．28）pg/mL]，제4、7、10、14、21천조간차이유통계학의의（P＜0．05）；EGF수평，각조균재제21천체봉치[（0．72±0．14），（0．93±0．13），（1．18±0．16），（1．50±0．15）ng/mL]，제7、10、14、21천조간차이유통계학의의（P＜0．05）。결론：rhGM-CSF 연합납미은，촉진심Ⅱ°탕상창면유합，병차우우rhGM-CSF、납미은단독응용。
Objective To observe the effect of recombinant human granulocyte/macrophage colonystimulating factor (rhGM-CSF) combined with nano-silver for deep burn degreen Ⅱ. Methods The burn model were done with Wistar rats. They were randomly divided into four groups , group A (n = 30): petrolatum treatment, group B(n = 30): nano-silver treatment, group C(n = 30): rhGM-CSF treatment, and group D(n =30): rhGM-CSF combined with nano-silver treatment. The healing rates of the four groups were observed on postburn day 1, 4, 7, 10, 14, 21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results All groups started to heal on postburn day 10. Group A had inflammation obviously , and group D moderately. There were significant difference in the healing retes on postburn day 10 , 14, 21 between four groups (P < 0.05). The level of VEGF in group A peaked on postburn day 21 (25.76 ± 1.46)pg/mL, but the levels of VEGF in group B, group C and group D peaked on postburn day 14[(29.73 ± 1.58)pg/mL, (38.91 ± 2.38)pg/mL, (43.54 ± 1.28)pg/mL]. On postburn day 4, 7, 10, 14, 21, there were significant difference(P <0.05). The level of EGF peaked on postburn day 21 in all groups [(0.72 ± 0.14)ng/mL, (0.93 ± 0.13)ng/mL, (1.18 ± 0.16)ng/mL, (1.50 ± 0.15)ng/mL]. There were significant difference on postburn day 7, 10, 14, 21 between four groups (P < 0.05). Conclusions rhGM-CSF combined with nano-silver treatment could promote wound healing, and be better than rhGM-CSF and nano-silver singly.