CAJ | 학술논문

目的：检测神经突蛋白（neuritin）在大鼠创伤性脑损伤组织中的时相性表达，探讨其在脑损伤后的意义。方法：42只 SD 大鼠分为正常组、对照组和实验组，改良 Feeney 法建立大鼠脑损伤模型，术后分6 h、12 h、24 h、3 d、7 d、14 d 共6个亚组。免疫组化、Western-blot 检测脑损伤组织中 neuritin 蛋白表达。结果：免疫组化：实验组neuritin蛋白6 h 呈阳性表达，12 h～7 d 呈强阳性，明显高于对照组与正常组，14 d呈弱阳性表达。 Western-blot：实验组neuritin蛋白6 h稍增高，24 h达高峰，持续到7 d，与对照组、正常组比较差异均有统计学差异（P＜0．01），14 d明显降低，较正常组差异仍有统计学意义（P＜0．05）。结论：Neuritin蛋白在脑损伤组织中表达明显增高，可能在脑损伤后起着重要作用。
목적：검측신경돌단백（neuritin）재대서창상성뇌손상조직중적시상성표체，탐토기재뇌손상후적의의。방법：42지 SD 대서분위정상조、대조조화실험조，개량 Feeney 법건립대서뇌손상모형，술후분6 h、12 h、24 h、3 d、7 d、14 d 공6개아조。면역조화、Western-blot 검측뇌손상조직중 neuritin 단백표체。결과：면역조화：실험조neuritin단백6 h 정양성표체，12 h～7 d 정강양성，명현고우대조조여정상조，14 d정약양성표체。 Western-blot：실험조neuritin단백6 h초증고，24 h체고봉，지속도7 d，여대조조、정상조비교차이균유통계학차이（P＜0．01），14 d명현강저，교정상조차이잉유통계학의의（P＜0．05）。결론：Neuritin단백재뇌손상조직중표체명현증고，가능재뇌손상후기착중요작용。
Objective To detect time-dependent change of neuritin expression in brain tissues after traumatic brain injury and discuss the effect of neuritin after brain damage occurred. Methods Forty-two rats were divided into normal group, control and experimental group. According to the postoperative time divided into 6 subgroups, including 6 hours group, 12 hours group, 24 hours group, 3 days group, 7 days group and 14 days group. Immunohistochemical and western-blot were used to detected the protein expression levels of neuritin. Results The immunohistochemical staining indicated that the positive expression of neuritin was strong in the cytomembrane and cytoplasms of the neurons, with a higher intensity, 6 hours after the operation. 12 hours after the operation last to the seventh day, the neurons with the strongest positive expression, is significantly higher than control group and normal group, significant decrease on the fourteenth day. The result of western-blot indicated that the level of neuritin protein sharply increased at 6 hours, reached the peak on 24 hours and after lasted to the seventh day, significantly higher than control group and normal group (P < 0.01), significant decrease on the fourteenth day (P < 0.05). Conclusion Up-regulation of neuritin in cerebral contusion tissues may play an important role after traumatic brain injury.