CAJ | 학술논문

目的：探讨大黄素对CT26结肠癌小鼠调节性T细胞免疫抑制功能的影响。方法：CT26结肠癌小鼠分为阴性对照组、大黄素治疗组和荷瘤模型组，给药14 d ，分别检测以下指标：小鼠不同组织 T 细胞IFN-γ的分泌、效应细胞CD8＋CD3＋T细胞的比例、血清细胞因子 IFN-γ、TNF-β1、IL-10的水平、不同组织CD4＋CD25＋Tregs IL-10的分泌。结果：大黄素明显升高CD8＋CD3＋T细胞在肿瘤局部的比例（P＜0．05）；大黄素显著提高外周血和淋巴结中T细胞分泌 IFN-γ的能力（P＜0．05）；大黄素显著降低 IFN-γ、TNF-β1、IL-10在血清中的水平（P＜0．01）；大黄素有抑制CD4＋CD25＋Tregs分泌 IL-10的作用（P＜0．01）。结论：大黄素可能通过影响Tregs的免疫抑制功能发挥抗肿瘤的作用。
목적：탐토대황소대CT26결장암소서조절성T세포면역억제공능적영향。방법：CT26결장암소서분위음성대조조、대황소치료조화하류모형조，급약14 d ，분별검측이하지표：소서불동조직 T 세포IFN-γ적분비、효응세포CD8＋CD3＋T세포적비례、혈청세포인자 IFN-γ、TNF-β1、IL-10적수평、불동조직CD4＋CD25＋Tregs IL-10적분비。결과：대황소명현승고CD8＋CD3＋T세포재종류국부적비례（P＜0．05）；대황소현저제고외주혈화림파결중T세포분비 IFN-γ적능력（P＜0．05）；대황소현저강저 IFN-γ、TNF-β1、IL-10재혈청중적수평（P＜0．01）；대황소유억제CD4＋CD25＋Tregs분비 IL-10적작용（P＜0．01）。결론：대황소가능통과영향Tregs적면역억제공능발휘항종류적작용。
Objective To investigate the effect of emodin on immune suppression function of regulatory T cells in a mouse model of CT26 colon cancer. Methods Twenty-four mice were divided into the negative control group, the emodin group and the tumor group. The populations of CD8+CD3+T cells, the T cells producing IFN-γand the CD4+CD25+Tregs secreting IL-10 in different mouse tissues were detected by flow cytometry. Levels of IFN-γ, TNF- β1 and IL-10 in serum were determined by ELISA. Results Emodin could significantly increase the percent of CD8+CD3+T cells in tumor (P < 0.05) and improve the ability of IFN-γ secretion in T cells from peripheral blood and lymph nodes (P < 0.05). Emodin could reduce the levels of IFN-γ, TNF-β1 and IL-10 in the serum (P < 0.01) and inhibit IL-10 secretion in CD4+CD25+ Tregs (P < 0.01). Conclusion Emodin possesses the antitumor effect by affecting the immunosuppressive function of Tregs cells.