CAJ | 학술논문

目的：检测支气管哮喘（简称哮喘）急性发作期患者治疗前后血清白细胞介素20（IL-20）水平的变化，探讨IL-20在哮喘发病机制中的作用和临床意义。方法：选择2013年5-10月广西医科大学第一医院呼吸内科门诊收治的轻至中度急性发作哮喘患者45例作为哮喘组，体检中心的32例健康体检者作为对照组。哮喘组患者均接受沙美特罗替卡松50/250μg/μg（1吸，2次/d）、万托林（必要时）治疗，中度哮喘组加用茶碱缓释片，总疗程1周。分别检测哮喘组治疗前后及对照组血清IL-20的水平。同时检测免疫球蛋白E （IgE）、嗜酸粒细胞（EOS）数量、巨噬细胞趋化蛋白 ECP 及肺功能[FEV1占预计值百分比（FEV1％）]等，比较哮喘组治疗前后IL-20水平的变化及其与对照组之间的差异，分析其与IgE、EOS、ECP 和FEV1％等指标的相关性。结果：轻度和中度哮喘组治疗前血清IL-20水平[（23．43±13．60）pg/L和（33．78±22．69）pg/L]均高于对照组[（13．58±6．17）pg/L]，差异有统计学意义，中度组高于轻度组。轻度和中度哮喘组治疗后血清IL-20分别为（15．73±8．27）pg/L和（19．64±11．69）pg/L均低于治疗前，差异有统计学意义。 Pearson相关分析显示，IL-20与IgE，EOS和ECP呈正相关，而与FEVl％呈负相关。结论：IL-20可能参与了支气管哮喘发病过程。
목적：검측지기관효천（간칭효천）급성발작기환자치료전후혈청백세포개소20（IL-20）수평적변화，탐토IL-20재효천발병궤제중적작용화림상의의。방법：선택2013년5-10월엄서의과대학제일의원호흡내과문진수치적경지중도급성발작효천환자45례작위효천조，체검중심적32례건강체검자작위대조조。효천조환자균접수사미특라체잡송50/250μg/μg（1흡，2차/d）、만탁림（필요시）치료，중도효천조가용다감완석편，총료정1주。분별검측효천조치료전후급대조조혈청IL-20적수평。동시검측면역구단백E （IgE）、기산립세포（EOS）수량、거서세포추화단백 ECP 급폐공능[FEV1점예계치백분비（FEV1％）]등，비교효천조치료전후IL-20수평적변화급기여대조조지간적차이，분석기여IgE、EOS、ECP 화FEV1％등지표적상관성。결과：경도화중도효천조치료전혈청IL-20수평[（23．43±13．60）pg/L화（33．78±22．69）pg/L]균고우대조조[（13．58±6．17）pg/L]，차이유통계학의의，중도조고우경도조。경도화중도효천조치료후혈청IL-20분별위（15．73±8．27）pg/L화（19．64±11．69）pg/L균저우치료전，차이유통계학의의。 Pearson상관분석현시，IL-20여IgE，EOS화ECP정정상관，이여FEVl％정부상관。결론：IL-20가능삼여료지기관효천발병과정。
Objective To detect the pre- and post-treatment levels of serum interleukin (IL-20) in asthmatic patients during the acute exacerbation period and to investigate the role of IL-20 in the pathogenesis of bronchial asthma and relative clinical significance. Methods Forty-five cases of mild to moderate asthma outpatients in the First Affiliated Hospital of Guangxi Medical University from May 2013 to October 2013 were chosen as the asthma group and 32 healthy people who underwent routine physical examination as the control group. All the patients were treated with inhalation salmeterol and fluticasone 50/250 μg/μg (sucking twice a day) together with ventolin if necessary , and even with theophylline sustained-release tablets as the additional treatment in moderate asthma group for 1 week. The serum levels of IL-20 as well as immunoglobulin E (IgE) were detected by ELISA in the asthma group before and after treatment as well as in the control group. Other data including ECP , the number of eosinophil ceils (EOS) and the pulmonary function (FEV1%) were detected as well. The differences of IL-20 levels between the asthma group before and after treatment and the control group were analyzed and the correlation between IL-20 and Ig E, EOS, ECP and FEV1% were analyzed. Results Compared with the healthy control group [(13.58 ± 6.17)pg/L], the levels of IL-20 in the mild and moderate asthma pretreatment group were significantly increased [(23.43 ± 13.60)pg/L and (33.78 ± 22.69)pg/L]. Compared with the mild asthma group, the levels of IL-20 in the moderate asthma pretreatment group were significantly higher. After treatment , the levels of IL-20 [(15.73 ± 8.27)pg/L and (19.64 ± 11.69)pg/L] were decreased respectively in the asthmatic patients. The Pearson correlative analysis showed that there were positive correlations between the levels of 1L-20 and IgE , EOS and ECP respectively and there were negative correlations between IL-20 levels and FEV1%. Conclusion IL-20 may be involved in the pathogenesis of asthma.