CAJ | 학술논문

目的：探讨凋亡相关基因BAK、cFLIP在EMs组织中的表达情况及其生物学行为的相关关系。方法：采用免疫组化的方法检测40例正常子宫内膜、40例卵巢EMs 患者的在位内膜及异位内膜中的BAK、cFLIP基因蛋白的表达情况。结果：BAK、cFLIP基因蛋白在三组子宫内膜细胞都有表达； BAK基因蛋白在异位内膜组、在位内膜组及正常内膜组阳性表达率及阳性表达强度呈递增表达，而cFLIP呈递减表达，差异有显著性（P＜0．05）；分泌期正常内膜组BAK 基因蛋白阳性表达率及阳性表达强度显著高于增殖期，而cFLIP则相反（P＜0．05），在位内膜组、异位内膜组的增殖期与分泌期的BAK、cFLIP基因蛋白表达差异无显著性（P ＞0．05）；异位内膜组和在位内膜组中 BAK 基因蛋白表达率及阳性表达强度重度组（Ⅲ～Ⅳ期）明显低于轻度组（Ⅰ～Ⅱ期），而cFLIP的表达则相反（P＜0．05）。异位内膜组织中BAK与cFLIP表达水平呈负相关（r＝-0．389，P＜0．05）。结论：BAK、cFLIP的负相互作用，参与了EMs 凋亡与增殖紊乱过程，在EMs的诊断和治疗中具有重要意义。
목적：탐토조망상관기인BAK、cFLIP재EMs조직중적표체정황급기생물학행위적상관관계。방법：채용면역조화적방법검측40례정상자궁내막、40례란소EMs 환자적재위내막급이위내막중적BAK、cFLIP기인단백적표체정황。결과：BAK、cFLIP기인단백재삼조자궁내막세포도유표체； BAK기인단백재이위내막조、재위내막조급정상내막조양성표체솔급양성표체강도정체증표체，이cFLIP정체감표체，차이유현저성（P＜0．05）；분비기정상내막조BAK 기인단백양성표체솔급양성표체강도현저고우증식기，이cFLIP칙상반（P＜0．05），재위내막조、이위내막조적증식기여분비기적BAK、cFLIP기인단백표체차이무현저성（P ＞0．05）；이위내막조화재위내막조중 BAK 기인단백표체솔급양성표체강도중도조（Ⅲ～Ⅳ기）명현저우경도조（Ⅰ～Ⅱ기），이cFLIP적표체칙상반（P＜0．05）。이위내막조직중BAK여cFLIP표체수평정부상관（r＝-0．389，P＜0．05）。결론：BAK、cFLIP적부상호작용，삼여료EMs 조망여증식문란과정，재EMs적진단화치료중구유중요의의。
Objective To explore the association of expression of apoptosis-associated gene BAK and cFLIP with the biological behaviors in endometriosis. Methods The expression of BAK and cFLIP protein gene in eutopic and ectopic tissue samples from 40 cases with pathologically confirmed ovarian endometriosis and 40 cases with pathologically confirmed normal endometrium was detected by immunohistochemical method. Results ①The expression of BAK and cFLIP protein gene was found in three groups of different endometrial tissue. ②The expression of BAK protein gene was increased gradually in ectopic endometrial , eutopic endometrium and normal tissue and there was significantly difference between every two groups ,while cFLIP was contrary expressed (P <0.05); ③In normal endometrium, The expression of BAK protein gene in secretory phase was higher than that in proliferative phase (P < 0.05), while cFLIP was contrary expressed, in eutopic endometrium and ectopic endometrial tissue , The expression of BAK and cFLIP protein gene in secretive phase showed no statistically significant difierence with that in proliferative phase (P > 0.05). ④The expression of BAK protein gene in severe group (Ⅲ-Ⅳ period) is lower than mild group both in eutopic or ectopic endometrial tissues,while cFLIP was contrary expressed (P < 0.05). ⑤The expression of BAK and cFLIP was negatively correlated with each other in ectopic endometrium (r=-0.389,P< 0.05). Conclusion BAK and cFLIP was negatively expressed in EMS, which may take a part in the endometrial apoptosis and disorderly proliferation. BAK and cFLIP may play an important role in the the diagnosis and treatment of endometriosis.