CAJ | 학술논문

目的：探讨中国汉族人群载脂蛋白 C3（-482C＞T）多态性与非酒精性脂肪肝（nonalcoholic fatty liver disease， NAFLD）及其临床特征的关系。方法：采用聚合酶链式反应-限制性片段长度多态性，结合聚丙烯酰胺凝胶电泳技术分析300例NAFLD患者及300例健康对照者载脂蛋白C3（-482C＞T ）多态性。应用Logistic 回归模型分析该位点多态性对NAFLD及其临床特征的影响。结果：载脂蛋白C3（-482C＞T ）基因型频率在NAFLD患者及健康对照人群间差异无统计学意义（P＞0．05）。与野生纯合子CC相比，NAFLD患者T等位基因携带者更容易合并胰岛素抵抗、高血压、高甘油三酯血症或高密度脂蛋白胆固醇降低。结论：载脂蛋白C3（-482C＞T ）多态性与中国汉族人NAFLD易感性不相关，但T等位基因携带者增加NAFLD患者合并代谢异常的风险。
목적：탐토중국한족인군재지단백 C3（-482C＞T）다태성여비주정성지방간（nonalcoholic fatty liver disease， NAFLD）급기림상특정적관계。방법：채용취합매련식반응-한제성편단장도다태성，결합취병희선알응효전영기술분석300례NAFLD환자급300례건강대조자재지단백C3（-482C＞T ）다태성。응용Logistic 회귀모형분석해위점다태성대NAFLD급기림상특정적영향。결과：재지단백C3（-482C＞T ）기인형빈솔재NAFLD환자급건강대조인군간차이무통계학의의（P＞0．05）。여야생순합자CC상비，NAFLD환자T등위기인휴대자경용역합병이도소저항、고혈압、고감유삼지혈증혹고밀도지단백담고순강저。결론：재지단백C3（-482C＞T ）다태성여중국한족인NAFLD역감성불상관，단T등위기인휴대자증가NAFLD환자합병대사이상적풍험。
Objective To investigate the relation between apolipoproteinC3 (-482C>T ) polymorphism and nonalcoholic fatty liver disease (NAFLD) and its clinical characteristics in the Han Chinese population. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide gel electrophoresis(PAGE)were used to analyse the genotype of the apolipoproteinC3 (-482C>T) variants. Results No relation between the apolipopreoteinC3 (-482C>T) polymorphism and NAFLD was found. However, NAFLD patients carrying T allele were more susceptible to insulin resistant (IR), hypertension, hypertriglyceridemia, low levels of high-density lipoprotein cholesterol (HDL) than homozygote CC genotype. Conclusion There was no relation between the apolipopreoteinC3 (-482C>T)polymorphism and NAFLD in Han Chinese population, but T-carriers were more susceptible to metabolic disorder.