CAJ | 학술논문

目的：应用原子力显微镜（atomic force microscope， AFM）研究顺铂对肝癌HepG2细胞的作用，在纳米级水平上分析顺铂诱导HepG2细胞凋亡的超微结构变化。方法：顺铂处理HepG2细胞24 h、48 h 后，通过AFM分析细胞表面形貌和超微结构变化；同时，MTT法检测细胞的增殖抑制率，流式细胞术检测细胞凋亡率。结果：AFM检测到随着顺铂作用时间延长，HepG2细胞形变程度加深；细胞膜表面形成的凹陷和孔洞增多；细胞膜表面粒径大小、高低差（Rp-v）、平均粗糙度（Ra）、均方根粗糙度（Rq）和平均高度（Meant Ht）均显著增加；细胞增殖抑制率及凋亡率明显升高。结论：顺铂使HepG2细胞形态皱缩、细胞膜表面孔洞形成及粗糙度增加，进而诱导细胞的凋亡。
목적：응용원자력현미경（atomic force microscope， AFM）연구순박대간암HepG2세포적작용，재납미급수평상분석순박유도HepG2세포조망적초미결구변화。방법：순박처리HepG2세포24 h、48 h 후，통과AFM분석세포표면형모화초미결구변화；동시，MTT법검측세포적증식억제솔，류식세포술검측세포조망솔。결과：AFM검측도수착순박작용시간연장，HepG2세포형변정도가심；세포막표면형성적요함화공동증다；세포막표면립경대소、고저차（Rp-v）、평균조조도（Ra）、균방근조조도（Rq）화평균고도（Meant Ht）균현저증가；세포증식억제솔급조망솔명현승고。결론：순박사HepG2세포형태추축、세포막표면공동형성급조조도증가，진이유도세포적조망。
Objective To study the effect of cisplatin on the hepatocellular carcinoma HepG2 cells by Atomic Force Microscopy (AFM), then analysis the changes of ultrastructural in HepG2 cells during apoptosis induced by cisplatin at nanoscale level. Methods HepG2 cells were treated with cisplatin for 24h and 48h. The ultrastructural change of cell surface was detected by AFM , the inhibitory rate and apoptotic rate of cell were examined by MTT and fow cytometry. Results AFM images showed that with the prolongation of cisplatin-inducing, the deformation change of HepG2 cells varying degree. The cells appeared larger pore size of cell membrane, the value of cell membrane particle sizes, Rp-v, Ra, Rq and Meant Ht were significant increased. The inhibitory rate and apoptotic rate were significant increased. Conclusions Cisplatin induced shrinkage in cell morphology, pore formation and roughness increasing in cell membrane, thereby inducing apoptosis in HepG2 cells.