CAJ | 학술논문

目的：研究高张盐水对大鼠局灶性脑缺血再灌注损伤后 TNF-α、IL-1β、S-100β的影响，探讨高张盐水在脑缺血再灌注损伤中的作用。方法：清洁级雄性SD大鼠60只，随机分为4组。 A 组：假手术组；B组：缺血再灌注组；C组：4．2％NaCl 组；D组：高张盐水组。采用线栓法制作局灶性脑缺血再灌注模型，恢复脑血流1．5 min时，经股静脉注射4 mL/kg的4．2％NaCl（C组）或7．5％NaCl（D组）， A 组和B组不给任何药物。再灌注22 h后，进行神经功能障碍评分，取缺血侧顶叶附近脑皮质测TNF-α、IL-1β、S-100β的含量。结果：经高张盐水后处理后，脑组织TNF-α、IL-1β、S-100β含量明显降低，但仍较假手术组高，C组和D组与B组比较有显著差异（P＜0．05）。结论：高张盐水可减少缺血再灌注后脑组织TNF-α、IL-1β、S-100β含量。
목적：연구고장염수대대서국조성뇌결혈재관주손상후 TNF-α、IL-1β、S-100β적영향，탐토고장염수재뇌결혈재관주손상중적작용。방법：청길급웅성SD대서60지，수궤분위4조。 A 조：가수술조；B조：결혈재관주조；C조：4．2％NaCl 조；D조：고장염수조。채용선전법제작국조성뇌결혈재관주모형，회복뇌혈류1．5 min시，경고정맥주사4 mL/kg적4．2％NaCl（C조）혹7．5％NaCl（D조）， A 조화B조불급임하약물。재관주22 h후，진행신경공능장애평분，취결혈측정협부근뇌피질측TNF-α、IL-1β、S-100β적함량。결과：경고장염수후처리후，뇌조직TNF-α、IL-1β、S-100β함량명현강저，단잉교가수술조고，C조화D조여B조비교유현저차이（P＜0．05）。결론：고장염수가감소결혈재관주후뇌조직TNF-α、IL-1β、S-100β함량。
Objective To investigate the effect of hypertonic saline on TNF-а, IL-1β, S-100β level following focal cerebral ischemia-reperfusion injury in rats and explore the mechanisms involved. Methods Ninety rats were randomized equally into 4 groups,namely the pseudo-operated group (A group),untreated IR injury group (B group),C group (4.2% hypertonic saline) and D group (7.5% hypertonic saline). Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments in group C and D. After 22 h of reperfusion , TNF-а, IL-1β, S-100βexpression in the ischemic brain tissue were measured by enzyme-linked immunosorbent assay (ELISA). Results After hypcrtonic saline treatment, the two saline-treated groups showed significant reduction in TNF-а、IL-1β、S-100β levels , but were still higher than pseudo-operated group(A group). Compared with B group, significant difference can be seen among C and D group. Conclusion Hypcrtonic saline can reduce cerebral TNF-а、IL-1β、S-100βlevel of isehemia-reperfusion injury.