国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
15期
2063-2064
,共2页
高效液相色谱法%血浆%氨基酸对映体
高效液相色譜法%血漿%氨基痠對映體
고효액상색보법%혈장%안기산대영체
high performance liquid chromatography%plasma%amino acid enantiomers
目的：用反相高效液相色谱法探讨原发性肝癌患者L-苏氨酸（L-threonine ，L-Thr）、D-苏氨酸（D-threonine ，D-Thr）、L-丙氨酸（L-alanine ，L-Ala）和D-丙氨酸（D-alanine ，D-Ala）水平的变化。方法采用邻苯二甲醛和N-乙酰-L-半胱氨酸柱前衍生用反相色谱柱进行分离，流动相为20 mmol/L磷酸二氢钠溶液、乙腈梯度洗脱，流速1．0 mL/min ，激发波长350 nm ，发射波长450 nm。结果该方法可以同时分离测定血液中苏氨酸、丙氨酸氨基酸对映体，回归方程线性相关性良好，检测线分别为11．4 pg/mL、9．77 pg/mL、4．11 pg/mL和3．50 pg/mL ，回收率为90．22％～105．7％，日间、日内相对标准偏差（RSD）为1．04％～4．11％。结论该方法准确、灵敏、适用于临床和科研。
目的：用反相高效液相色譜法探討原髮性肝癌患者L-囌氨痠（L-threonine ，L-Thr）、D-囌氨痠（D-threonine ，D-Thr）、L-丙氨痠（L-alanine ，L-Ala）和D-丙氨痠（D-alanine ，D-Ala）水平的變化。方法採用鄰苯二甲醛和N-乙酰-L-半胱氨痠柱前衍生用反相色譜柱進行分離，流動相為20 mmol/L燐痠二氫鈉溶液、乙腈梯度洗脫，流速1．0 mL/min ，激髮波長350 nm ，髮射波長450 nm。結果該方法可以同時分離測定血液中囌氨痠、丙氨痠氨基痠對映體，迴歸方程線性相關性良好，檢測線分彆為11．4 pg/mL、9．77 pg/mL、4．11 pg/mL和3．50 pg/mL ，迴收率為90．22％～105．7％，日間、日內相對標準偏差（RSD）為1．04％～4．11％。結論該方法準確、靈敏、適用于臨床和科研。
목적：용반상고효액상색보법탐토원발성간암환자L-소안산（L-threonine ，L-Thr）、D-소안산（D-threonine ，D-Thr）、L-병안산（L-alanine ，L-Ala）화D-병안산（D-alanine ，D-Ala）수평적변화。방법채용린분이갑철화N-을선-L-반광안산주전연생용반상색보주진행분리，류동상위20 mmol/L린산이경납용액、을정제도세탈，류속1．0 mL/min ，격발파장350 nm ，발사파장450 nm。결과해방법가이동시분리측정혈액중소안산、병안산안기산대영체，회귀방정선성상관성량호，검측선분별위11．4 pg/mL、9．77 pg/mL、4．11 pg/mL화3．50 pg/mL ，회수솔위90．22％～105．7％，일간、일내상대표준편차（RSD）위1．04％～4．11％。결론해방법준학、령민、괄용우림상화과연。
Objective To investigate the change of plasma L-threonine (L-Thr) ,D-threonine (D-Thr) ,L-alanine (L-Ala) and D-alanine (D-Ala) in the patients with primary hepatocellular carcinoma (HCC) by using the reversed-phase high-performance liquid chromatography (RP-HPLC) .Methods The o-phthaldialdehyde (OPA) and N-acetyl-L-cysteine (NAC) column with pre-column derivatization was adopted to perform the separation .The mobile phase was 20 mmol/L sodium dihydrogen phosphate solution and acetonitrile with a programmed gradient elution and the flow rate of 1 .0 mL/min .The excitation wavelength was set at 350 nm and the emission wavelength was set at 450 nm .Results The threonine and alanine enantiomers could be simultaneously determined by this method .The regression equation showed the good linear correlation The limits of detection (LOD) for L-Thr ,D-Thr ,L-Ala and D-Ala were 11 .4 pg/mL ,9 .77 pg/mL ,4 .11 pg/mL and 3 .50 pg/mL respectively .The recovery rate was 90 .22% -105 .7% .With-in-day and between-day RSD was 1 .04% -4 .11% .Conclusion The threonine and alanine enantiomers could be simultaneously de-termined by this method .The regression equation showed the good linear correlation The limits of detection (LOD) for L-Thr ,D-Thr ,L-Ala and D-Ala were 11 .4 pg/mL ,9 .77 pg/mL ,4 .11 pg/mL and 3 .50 pg/mL respectively .The recovery rate was 90 .22%-105 .7% .Within-day and between-day RSD was 1 .04% -4 .11% .
