CAJ | 학술논문

目的：评价两种方法对蒙药金诃子低温提取物多糖成分和含量的检测效果。方法采用红外光谱技术和硫酸-蒽酮比色法测定蒙药金诃子5个不同部位提取物中多糖的成分和含量，红外光谱技术检测部分由清华大学分析中心完成。评价硫酸-蒽酮比色法的精密度、稳定性、重复性和加样回收率等性能指标。结果红外光谱技术测得蒙药金诃子5个部位提取物中均有多糖成分，但各部位的含量比较差异无统计学意义。硫酸-蒽酮比色法线性范围为40～200μg/mL；精密度良好（ RSD＝0．460％），重复性良好（ RSD＝1．616％），平均加标回收率为（110．130±0．010）％（ RSD＝0．946％）；试剂在室温自然光下2 h内稳定性良好；标准曲线线性回归方程为Y＝0．2051X-0．0034，R＝0．9991；蒙药金诃子5个部位提取物中多糖含量为14．15％～22．15％。结论蒙药金诃子不同部位提取物的成分分析优选红外光谱技术，而硫酸-蒽酮比色法用于检测多糖含量。
목적：평개량충방법대몽약금가자저온제취물다당성분화함량적검측효과。방법채용홍외광보기술화류산-은동비색법측정몽약금가자5개불동부위제취물중다당적성분화함량，홍외광보기술검측부분유청화대학분석중심완성。평개류산-은동비색법적정밀도、은정성、중복성화가양회수솔등성능지표。결과홍외광보기술측득몽약금가자5개부위제취물중균유다당성분，단각부위적함량비교차이무통계학의의。류산-은동비색법선성범위위40～200μg/mL；정밀도량호（ RSD＝0．460％），중복성량호（ RSD＝1．616％），평균가표회수솔위（110．130±0．010）％（ RSD＝0．946％）；시제재실온자연광하2 h내은정성량호；표준곡선선성회귀방정위Y＝0．2051X-0．0034，R＝0．9991；몽약금가자5개부위제취물중다당함량위14．15％～22．15％。결론몽약금가자불동부위제취물적성분분석우선홍외광보기술，이류산-은동비색법용우검측다당함량。
Objective To estimate the detection results of the two methods on the determination of components and con -tent of polysaccharides in low temperature extracts from Mongolian Terminalia chebula Ritz .Methods The components and content of polysaccharides in five different positions were determined by infrared spectroscopy and anthrone -sulfuric acid color-imetric method.The infrared spectroscopy detection was completed in Tsinghua university analysis center .The precision, sta-bility, repeatability and average recovery rate of anthrone-sulfuric acid colorimetric method were evaluated by different experi-ments.Results The polysaccharides in the five different positions were determined by infrared spectroscopy , but no signifi-cant difference was found among the five different positions .In anthrone-sulfuric acid colorimetric method , the linear ranges of polysaccharides were 40-200 μg/mL, the precision was good ( RSD =0.460%) , the repeatability was good ( RSD =1.616%), and the average recovery rate was (110.130 ±0.010)%(RSD=0.946%).The reagent stability was good in 2 h at room temperature under natural light; linear regression equation of standard curve was Y =0.205 1X-0.003 4, R=0.999 1.There were polysaccharides in the five different positions , and the contents of polysaccharides were 14.15%-22.15%.Conclusions The component analysis of polysaccharides in the five different positions is preferably detected by in -frared spectroscopy , and anthrone-sulfuric acid colorimetric method is used to determine the contents of polysaccharides .