CAJ | 학술논문

目的：建立离体和在体机械损伤致血管平滑肌增殖模型。方法原代培养大鼠胸主动脉血管平滑肌细胞（VSMCs），体外建立机械划伤致VSMCs增殖模型，采用5-溴脱氧尿嘧啶（5-BrUd）掺入法，流式细胞术检测细胞增殖情况；整体动物上，采用新西兰兔左侧颈总动脉球囊损伤，术后7d取颈总动脉段，常规病理切片，HE染色，观测血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积。结果机械划痕损伤12 h后划伤处两侧的细胞开始有部分增殖和迁移，24 h后细胞增殖较为明显。流式细胞术检测机械损伤后细胞增殖增多（P＜0.05）。整体动物上，与假手术组比较，模型组术后7 d血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积显著增加（P＜0.01）。结论机械损伤可以导致离体和在体血管平滑肌细胞增殖。
목적：건립리체화재체궤계손상치혈관평활기증식모형。방법원대배양대서흉주동맥혈관평활기세포（VSMCs），체외건립궤계화상치VSMCs증식모형，채용5-추탈양뇨밀정（5-BrUd）참입법，류식세포술검측세포증식정황；정체동물상，채용신서란토좌측경총동맥구낭손상，술후7d취경총동맥단，상규병리절편，HE염색，관측혈관내막후도、내막면적、내막후도/중막후도、내막면적/중막면적。결과궤계화흔손상12 h후화상처량측적세포개시유부분증식화천이，24 h후세포증식교위명현。류식세포술검측궤계손상후세포증식증다（P＜0.05）。정체동물상，여가수술조비교，모형조술후7 d혈관내막후도、내막면적、내막후도/중막후도、내막면적/중막면적현저증가（P＜0.01）。결론궤계손상가이도치리체화재체혈관평활기세포증식。
Objective To establish the proliferation model of vascular smooth muscle cells induced by mechanical injury in vitro and in vivo. Methods The primarily cultured rat thoracic aortic SMCs were used to establish mechanical injury models in vitro. The proliferation activity of VSMCs was analyzed by flow cytometry with 5-BrUd incorporation. The proliferation of vascular smooth muscle cells (VSMCs) in vivo was established by balloon injury on the rabbit carotid artery. After 7 days, experimental segments of arteries were harvested and subsequently progressed routine pathological sections, hematoxylin-eosin staining. Results The proliferation and migration of VSMCs were observed after 12h mechanical injury, especially after 24h. Compared with the sham group, the proliferative cells were increased by mechanical injury by the analysis of flow cytometry(P<0.05). Tthe intimal thickness, intimal area, intimal/medial thickness and intima-to-media area ratio of the Model group significantly increase comparing to the sham group (P<0.01). Conclusion The proliferation model of vascular smooth muscle cells induced by mechanical injury in vitro and in vivo were successfully established.