CAJ | 학술논문

目的：观察白细胞介素(IL)-22对黑素细胞生物活性的影响，包括黑素细胞的增生、黑素细胞酪氨酸酶活性和黑素细胞凋亡，从而研究色素障碍性皮肤病的发病机制，为色素性疾病的治疗提供新的思路。方法建立模拟正常生理状态的黑素细胞体外培养体系，用不同浓度IL-22作用于黑素细胞，采用CCK-8法检测不同浓度IL-22对黑素细胞增生活性的影响；L-DOPA法检测其对黑素细胞酪氨酸酶活性的影响；流式细胞仪检测其对黑素细胞凋亡的影响。结果①IL-22随着浓度的增加呈明显的时间依赖性抑制黑素细胞的增生，与空白对照组相比差异有统计学意义（P＜0.05）。②用浓度为100 ng/ml的IL-22培养黑素细胞，48 h后细胞凋亡率为23.0%，空白对照组为5.1%。结论 IL-22随着浓度的增加明显抑制黑素细胞的增生，呈时间依赖性，当培养时间为72 h时，所有浓度均抑制黑素细胞的增生，黑素细胞酪氨酸酶活性也随着IL-22浓度的增加而降低，黑素细胞凋亡率较空白对照组明显升高，均提示IL-22可引起黑素细胞的损伤。
목적：관찰백세포개소(IL)-22대흑소세포생물활성적영향，포괄흑소세포적증생、흑소세포락안산매활성화흑소세포조망，종이연구색소장애성피부병적발병궤제，위색소성질병적치료제공신적사로。방법건립모의정상생리상태적흑소세포체외배양체계，용불동농도IL-22작용우흑소세포，채용CCK-8법검측불동농도IL-22대흑소세포증생활성적영향；L-DOPA법검측기대흑소세포락안산매활성적영향；류식세포의검측기대흑소세포조망적영향。결과①IL-22수착농도적증가정명현적시간의뢰성억제흑소세포적증생，여공백대조조상비차이유통계학의의（P＜0.05）。②용농도위100 ng/ml적IL-22배양흑소세포，48 h후세포조망솔위23.0%，공백대조조위5.1%。결론 IL-22수착농도적증가명현억제흑소세포적증생，정시간의뢰성，당배양시간위72 h시，소유농도균억제흑소세포적증생，흑소세포락안산매활성야수착IL-22농도적증가이강저，흑소세포조망솔교공백대조조명현승고，균제시IL-22가인기흑소세포적손상。
Objective To observe the effects of IL-22 on biological activity of human epidermal melanocytes, including proliferation, tyrosinase activity, and apoptosis of melanocytes. The results of the study would help us to better understand the pathogenesis of pigment aplastic skin diseases and provide new ideas for the treatment of pigment disorders. Methods An in vitro culture system of melanocytes simulated normal physiological state was established, then the effects of IL-22 of different concentrations on proliferative activity of human epidermal melanocytes were detected by CCK-8 method, meanwhile the effects on tyrosinase activity were detected by L-DOPA method, and the effects on apoptosis were detected by lfow cytometry. Results With the increase of concentration, IL-22 exhibited time-dependent inhibitory effect on the proliferation of melanocytes, for the inhibitory ratio of melanocytes, there was signiifcant difference between the IL-22 groups and the control group (P<0.05). For the melanocytes cultured at the concentration of 100 ng/ml of IL-22 and the control group, the apoptosis rate of melanocytes were 23.0%and 5.1%respectively after 48 h of cell culture. Conclusions With the increase of concentration, IL-22 exhibited time-dependent inhibitory effect on the proliferation of melanocytes. When the culture time over 72 hours, the proliferation of melanocytes was inhibited in IL-22 groups with all the different concentrations; meanwhile with the increase of the concentration of IL-22, the tyrosinase activity of melanocytes gradually decreased;compared with the control group, the apoptosis rate of melanocytes in IL-22 groups increased signiifcantly. All these results suggest that IL-22 may cause damage of melanocytes.