CAJ | 학술논문

目的：探讨盐酸替罗非班（tirofiban hydrochloride ，TH）在兔心肌缺血再灌注（ischemia reperfusion ，IR）损伤中的作用及其机制。方法：30只雄性新西兰大白兔随机分为3组：假手术组（S组）、心肌I/R模型组（I/R组）和心肌 I/R＋盐酸替罗非班治疗组（I/R＋ T H组），每组10只。采用结扎冠状动脉前降支1h后恢复血供的方法制备心肌I/R损伤模型，再灌注0、1、2、4h 分别收集血样，酶联免疫吸附法（ELISA）测定血清中肌钙蛋白 T（TnT）及炎症因子（IL-32、TNF-α及IL-1β）的水平，再灌注后4h处死动物，化学比色法检测心脏组织中髓过氧化物酶（MPO）的活性。结果：TnT 水平，再灌注4h ，I/R、I/R＋ TH组均较S组显著升高（P＜00．5）；炎症因子水平，缺血再灌注后I/R、I/R＋ TH 组均较S组显著升高（P＜00．5），I/R＋ TH 组在再灌注1、2h 有明显改善，组间两两比较，差异有统计学意义（P＜00．5）；MPO活性，与S组比较，I/R组显著增强（P＜0．05），I/R＋ TH 组差异无统计学意义（P＞0．05）。结论：盐酸替罗非班对兔心肌I/R损伤具有保护作用，其机制与减少炎症因子释放、降低 M PO 活性，抑制中性粒细胞聚集有关。
목적：탐토염산체라비반（tirofiban hydrochloride ，TH）재토심기결혈재관주（ischemia reperfusion ，IR）손상중적작용급기궤제。방법：30지웅성신서란대백토수궤분위3조：가수술조（S조）、심기I/R모형조（I/R조）화심기 I/R＋염산체라비반치료조（I/R＋ T H조），매조10지。채용결찰관상동맥전강지1h후회복혈공적방법제비심기I/R손상모형，재관주0、1、2、4h 분별수집혈양，매련면역흡부법（ELISA）측정혈청중기개단백 T（TnT）급염증인자（IL-32、TNF-α급IL-1β）적수평，재관주후4h처사동물，화학비색법검측심장조직중수과양화물매（MPO）적활성。결과：TnT 수평，재관주4h ，I/R、I/R＋ TH조균교S조현저승고（P＜00．5）；염증인자수평，결혈재관주후I/R、I/R＋ TH 조균교S조현저승고（P＜00．5），I/R＋ TH 조재재관주1、2h 유명현개선，조간량량비교，차이유통계학의의（P＜00．5）；MPO활성，여S조비교，I/R조현저증강（P＜0．05），I/R＋ TH 조차이무통계학의의（P＞0．05）。결론：염산체라비반대토심기I/R손상구유보호작용，기궤제여감소염증인자석방、강저 M PO 활성，억제중성립세포취집유관。
Objective:To investigate the effect of tirofiban hydrochloride (TH) on myocardial ischemia reperfusion injury and its mechanism in rabbits .Methods :Thirty male New Zealand white rabbits were ran-domly divided into three groups :sham group(S group ,n=10) ,ischemia reperfusion group(I/R group ,n=10) and TH treatment group (I/R+ TH group ,n=10) .Myocardial ischemia-reperfusion was created by liga-ting the left anterior descending coronary artery for 1 hour followed by reperfusion .0 ,1 ,2 ,4 h after reperfu-sion ,the plasma was collected .Plasma levels of troponin T (TnT) ,IL-32 ,TNF-αand IL-1βwere measured using ELISA ,activity of myeloperoxidase in heart tissue by chemical chromatometry .Results:4 h after reperfusion ,Plasma levels of TnT in I/R ,I/R+ TH group were significantly higher than S group (P<0 . 05) ,Plasma levels of inflammatory factor in I/R ,I/R+ THgroup were significantly higher than S group af-ter ischemia reperfusion (P<0 0.5) ,1 ,2 h after reperfusion ,Plasma levels of inflammatory factor in I/R+TH group were lower significantly than befor reperfusion (0 h after reperfusion) ,the differences were statis-tically significant by pairwise comparison (P<0 .05) .Compared with the S group ,the activity of MPO re-duced significantly(P<0 .05) ,There was no significant difference in I/R+ TH group(P> 0 .05) .Conclu-sions:Tirofiban hydrochloride has protective effect on myocardial ischemia reperfusion injury in rabbits which associate with inflammatory cytokines decrease ,reduce MPO activity decrease ,and neutrophils aggre-gation inhibition .