CAJ | 학술논문

试验条件下研究了不同质量浓度（2 mg·L -1、8 mg·L -1和32 mg·L -1）双环芳烃---萘暴露对环文蛤（Cycli-na sinensis）超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、碱性磷酸酶（AKP）活性和丙二醛（MDA）生成量变化的影响。结果表明，高浓度萘（32 mg·L -1）在15 d 内对环文蛤有很强的致死效应；在非致死条件下，随暴露时间延长 SOD 活性持续升高，CAT 活力存在低浓度促进高浓度抑制的现象，AKP 活性随暴露时间延长表现出先升高后降低的趋势，而 MDA 生成量则随暴露时间延长呈现低→高→低的变化。Pearson 相关分析显示，在环文蛤应对氧化胁迫的过程中 SOD 和 CAT 呈现协同作用（R ＝0.439，P ﹤0.01），SOD 和 AKP 表现拮抗关系（R ＝-0.571，P ﹤0.01）。在保护细胞膜结构完整性方面，CAT 和 AKP 可能较 SOD 发挥更大作用（R ＝-0.490，P ﹤0.01）。
시험조건하연구료불동질량농도（2 mg·L -1、8 mg·L -1화32 mg·L -1）쌍배방경---내폭로대배문합（Cycli-na sinensis）초양화물기화매（SOD）、과양화경매（CAT）、감성린산매（AKP）활성화병이철（MDA）생성량변화적영향。결과표명，고농도내（32 mg·L -1）재15 d 내대배문합유흔강적치사효응；재비치사조건하，수폭로시간연장 SOD 활성지속승고，CAT 활력존재저농도촉진고농도억제적현상，AKP 활성수폭로시간연장표현출선승고후강저적추세，이 MDA 생성량칙수폭로시간연장정현저→고→저적변화。Pearson 상관분석현시，재배문합응대양화협박적과정중 SOD 화 CAT 정현협동작용（R ＝0.439，P ﹤0.01），SOD 화 AKP 표현길항관계（R ＝-0.571，P ﹤0.01）。재보호세포막결구완정성방면，CAT 화 AKP 가능교 SOD 발휘경대작용（R ＝-0.490，P ﹤0.01）。
The SOD,CAT,AKP activities and MDA content were determined after Cyclina sinensis were exposed to different concen-trations(2 mg·L - 1 ,8 mg·L - 1 and 32 mg·L - 1 )of naphthalene under experimental ecotoxicology conditions. The results indicate that C. sinensis had high mortality rate under 32 mg·L - 1 of naphthalene. Under non-lethal conditions and with increase of naphthalene expo-sure time,SOD activity showed a continuous increasing trend;CAT activity was promoted in low-concentration group and inhibited in high-concentration group;AKP activity was initially increasing then decreasing;MDA content fluctuated following the trend of low→high→low. According to Pearson correlation analysis,SOD and CAT showed synergistic effect(R = 0. 439,P ﹤ 0. 01),while SOD and AKP showed antagonism effect(R = - 0. 571,P ﹤ 0. 01). CAT and AKP might protect cellular membrane structure better than SOD(R = - 0. 490,P ﹤ 0. 01).