CAJ | 학술논문

细胞周期蛋白 B（Cyclin B）是影响卵母细胞发育成熟的重要基因。文章构建了 PmCyB 慢病毒表达载体，并和慢病毒包装复合物在293T 细胞中包装成慢病毒颗粒。利用包装好的慢病毒颗粒，感染干扰了 Cyclin B1基因的Hela 细胞，研究了 PmCyB 对 Hela 细胞增殖的影响。结果表明，通过 siRNA 干扰细胞自身的 Cyclin B1后，转染对照组病毒液的 Hela 细胞增殖减缓；同时，干扰后转入含有 PmCyB 重组慢病毒表达载体，Hela 细胞增殖速度明显要高于转染对照组病毒液的 Hela 细胞的增殖速度。这一结果表明，PmCyB 基因的过表达能补偿 Cyclin B1缺失导致的细胞增殖减缓的现象，说明 PmCyB 基因具有细胞周期调控功能，是细胞增殖与分化的重要调控基因，且功能可能与人类的 Cyclin B1相似。人类的 Cyclin B1是卵母细胞发育成熟的重要基因。由此可以推断，PmCyB 基因可能对斑节对虾（Penaeus monodon）卵巢发育成熟起着重要作用。
세포주기단백 B（Cyclin B）시영향란모세포발육성숙적중요기인。문장구건료 PmCyB 만병독표체재체，병화만병독포장복합물재293T 세포중포장성만병독과립。이용포장호적만병독과립，감염간우료 Cyclin B1기인적Hela 세포，연구료 PmCyB 대 Hela 세포증식적영향。결과표명，통과 siRNA 간우세포자신적 Cyclin B1후，전염대조조병독액적 Hela 세포증식감완；동시，간우후전입함유 PmCyB 중조만병독표체재체，Hela 세포증식속도명현요고우전염대조조병독액적 Hela 세포적증식속도。저일결과표명，PmCyB 기인적과표체능보상 Cyclin B1결실도치적세포증식감완적현상，설명 PmCyB 기인구유세포주기조공공능，시세포증식여분화적중요조공기인，차공능가능여인류적 Cyclin B1상사。인류적 Cyclin B1시란모세포발육성숙적중요기인。유차가이추단，PmCyB 기인가능대반절대하（Penaeus monodon）란소발육성숙기착중요작용。
Cyclin B plays an important role in oocyte development. The recombinant lentiviral vector of Penaeus monodon Cyclin B (PmCyB)was constructed. The recombinant lentiviral vector plasmid and Packaging Plasmid Mix were co-transfected into 293T cells to obtain recombinant lentivirus. After Cyclin B1 of Hela cells was interfered with siRNA,the recombinant lentivirus was transfected into these cells and its influence on the Hela cells proliferation was researched. The results show that Hela cells proliferation slowed down after siRNA interference,but renewed after being transfected by the packaged lentivirus particles containing PmCyB,which indicates that PmCyB gene has cell cycle activity and is important for regulation of cell proliferation and differentiation gene. The results also suggest that PmCyB might play an important role in ovarian development,as same as Homo sapiense Cyclin B1 which is an ovarian de-velopment essential gene.