成都体育学院学报
成都體育學院學報
성도체육학원학보
JOURNAL OF CHENGDU PHYSICAL EDUCATION INSTITUTE
2014年
8期
77-82
,共6页
L-肉碱%运动%CPT-1 酶%FAT/ CD36
L-肉堿%運動%CPT-1 酶%FAT/ CD36
L-육감%운동%CPT-1 매%FAT/ CD36
L-carnitine%exercise%CPT-1%FAT/ CD36
目的：研究补充 L-肉碱结合运动对营养型肥胖小鼠骨骼肌 FAT/ CD36以及 CPT-1的影响。方法：49只 C57BL/6雄性小鼠,随机选取9只喂养普通饲料(C 组),另外40只建立肥胖模型(H 组)。建模成功后将小鼠随机分为肥胖安静组(H)、肥胖肉碱组(HL)、肥胖运动组(HE)、肥胖肉碱结合运动组(HLE)。 HE、HLE 组进行为期5周的游泳训练,5天/周,1小时/天。 HL、HLE 组小鼠每天训练前1小时灌胃 L-肉碱。实验期间监测小鼠体重变化,实验结束后取腓肠肌检测相关指标。结果：①HE、 HLE 组体重低于 H 组,且 HLE 组体重低于 HL 组；②HE、HL 组和 HLE 组的 FFA 含量显著低于 H 组,而 HE、HLE 组的 FFA 含量也明显低于 HL组；③HL、HE 组和 HLE 组的 CPT-1酶含量显著低于 H 组；④HE 组的 FAT/ CD36表达量高于 H 组,HLE 组则低于 H 组；⑤CPT-1酶含量和 FAT/ CD36表达量与体重均呈中度正相关。以上差异均具有统计学意义,P<0.05或 P<0.01。结论：①与补充 L-肉碱相比,运动和运动结合 L-肉碱有更显著的控体重效果和促进脂肪酸氧化的作用。②单纯运动,补充 L-肉碱以及运动结合 L-肉碱均可降低骨骼肌内 CPT-1酶的含量。③运动结合左旋肉碱可交互促进降低 FAT/ CD36的表达量。
目的：研究補充 L-肉堿結閤運動對營養型肥胖小鼠骨骼肌 FAT/ CD36以及 CPT-1的影響。方法：49隻 C57BL/6雄性小鼠,隨機選取9隻餵養普通飼料(C 組),另外40隻建立肥胖模型(H 組)。建模成功後將小鼠隨機分為肥胖安靜組(H)、肥胖肉堿組(HL)、肥胖運動組(HE)、肥胖肉堿結閤運動組(HLE)。 HE、HLE 組進行為期5週的遊泳訓練,5天/週,1小時/天。 HL、HLE 組小鼠每天訓練前1小時灌胃 L-肉堿。實驗期間鑑測小鼠體重變化,實驗結束後取腓腸肌檢測相關指標。結果：①HE、 HLE 組體重低于 H 組,且 HLE 組體重低于 HL 組；②HE、HL 組和 HLE 組的 FFA 含量顯著低于 H 組,而 HE、HLE 組的 FFA 含量也明顯低于 HL組；③HL、HE 組和 HLE 組的 CPT-1酶含量顯著低于 H 組；④HE 組的 FAT/ CD36錶達量高于 H 組,HLE 組則低于 H 組；⑤CPT-1酶含量和 FAT/ CD36錶達量與體重均呈中度正相關。以上差異均具有統計學意義,P<0.05或 P<0.01。結論：①與補充 L-肉堿相比,運動和運動結閤 L-肉堿有更顯著的控體重效果和促進脂肪痠氧化的作用。②單純運動,補充 L-肉堿以及運動結閤 L-肉堿均可降低骨骼肌內 CPT-1酶的含量。③運動結閤左鏇肉堿可交互促進降低 FAT/ CD36的錶達量。
목적：연구보충 L-육감결합운동대영양형비반소서골격기 FAT/ CD36이급 CPT-1적영향。방법：49지 C57BL/6웅성소서,수궤선취9지위양보통사료(C 조),령외40지건립비반모형(H 조)。건모성공후장소서수궤분위비반안정조(H)、비반육감조(HL)、비반운동조(HE)、비반육감결합운동조(HLE)。 HE、HLE 조진행위기5주적유영훈련,5천/주,1소시/천。 HL、HLE 조소서매천훈련전1소시관위 L-육감。실험기간감측소서체중변화,실험결속후취비장기검측상관지표。결과：①HE、 HLE 조체중저우 H 조,차 HLE 조체중저우 HL 조；②HE、HL 조화 HLE 조적 FFA 함량현저저우 H 조,이 HE、HLE 조적 FFA 함량야명현저우 HL조；③HL、HE 조화 HLE 조적 CPT-1매함량현저저우 H 조；④HE 조적 FAT/ CD36표체량고우 H 조,HLE 조칙저우 H 조；⑤CPT-1매함량화 FAT/ CD36표체량여체중균정중도정상관。이상차이균구유통계학의의,P<0.05혹 P<0.01。결론：①여보충 L-육감상비,운동화운동결합 L-육감유경현저적공체중효과화촉진지방산양화적작용。②단순운동,보충 L-육감이급운동결합 L-육감균가강저골격기내 CPT-1매적함량。③운동결합좌선육감가교호촉진강저 FAT/ CD36적표체량。
Objective: To study the effects of L-carnitine combined with exercise on FAT/ CD36 and CPT-1 of obese mice. Methods: Male C57BL / 6 mice were randomly assigned to group C (fed with normal diet) and group H (fed with high-fat diet). After group H was modeled successfully, they were randomly divided into group H (obesity quiet), group HL (obesity with L-carnitine), group HE (obesity with exercise), group HLE (obesity with L-carnitine combined exercise). Swimming training was adopted to them, 1 hour / day, 5 days / week, lasting for five weeks. Group HL, HLE were gavaged with L-carnitine an hour before training. During the experiment, changes of body weight of mice were monitored. After the experiment, the gastrocnemius muscles were prepared to determine the content of FFA, CPT-1 and FAT/ CD36. Results: ① After 5 weeks of training, the body weight of group HE and HLE became lower than group H, and the weight of group HLE was also lower than group HL; ② the FFA of group HL, HE and HLE was significantly lower than in group H, and in group HE and HLE it was also significantly lower than group HL; ③ the contents of CPT-1 of group HL, HE and HLE were significantly lower than in group H; ④ the expression of FAT/ CD36 of group HE was higher than in group H, HLE group was lower than group H; ⑤ the content of CPT-1 and FAT/ CD36 showed a moderate positive correlation with body weight. These differences were statistically significant, P <0. 05 or P <0. 01. Conclusion: ① there is no apparent role of pure L-carnitine supplementation in controlling weight. However, there is significant effect of exercise and exercise combined with L-carnitine on controlling weight. ② the effect of exer- <br> cise and exercise combined with L-carnitine in promoting metabolism and oxidation of fatty acid is more obvious than pure L-carnitine supplementation. ③ L-carnitine, exercise, and exercise combined with L-carnitine can reduce CPT-1 levels. ④ exercise combined with L-carnitine can interactively reduce the expression of FAT/ CD36. The contents of CPT-1, FAT/ CD36 are moderately correlated with body weight.
