CAJ | 학술논문

在前期筛选具有抗猪繁殖与呼吸综合征病毒（ PRRSV）作用的中药研究基础上，选取药效较好的三种中药：穿心莲、芦根和夏枯草。为进一步研究其有效抗病毒成分，采用MTT 法观察了穿心莲内酯盐、芦根多糖和夏枯草多糖成分对猴肾上皮细胞系（ Marc-145）生长的影响，分别采用阻断、抑制、直接杀灭3种作用方式观察了各成分抗RP P SV感染作用。结果显示：穿心莲内酯盐对细胞最大安全浓度为0．16 mg/mL，芦根多糖为0．575 mg/mL，夏枯草多糖为1．125 mg/mL；芦根多糖在三种方式上均有不同程度的抗P RRSV作用，而穿心莲内酯盐和夏枯草多糖阻断P RRSV作用不明显，抑制和杀灭P RRSV作用明显。结果表明穿心莲内酯盐、芦根多糖和夏枯草多糖均有抗病毒作用，为抗病毒中药的主要药效成分。
재전기사선구유항저번식여호흡종합정병독（ PRRSV）작용적중약연구기출상，선취약효교호적삼충중약：천심련、호근화하고초。위진일보연구기유효항병독성분，채용MTT 법관찰료천심련내지염、호근다당화하고초다당성분대후신상피세포계（ Marc-145）생장적영향，분별채용조단、억제、직접살멸3충작용방식관찰료각성분항RP P SV감염작용。결과현시：천심련내지염대세포최대안전농도위0．16 mg/mL，호근다당위0．575 mg/mL，하고초다당위1．125 mg/mL；호근다당재삼충방식상균유불동정도적항P RRSV작용，이천심련내지염화하고초다당조단P RRSV작용불명현，억제화살멸P RRSV작용명현。결과표명천심련내지염、호근다당화하고초다당균유항병독작용，위항병독중약적주요약효성분。
Andrographolide, phragmites polysaccharide and selfheal polysaccharide were selected to study the antiviral action against to porcine reproductive and respiratory syndrome virus( PRRSV) in vitro. The blocking, inhibiting and killing roles of these extract were detected in Marc-145 cells infected with PRRSV. Results showed the safety concentration of andrographolide, phragmites polysaccharide and selfheal polysaccharide respectively are:0.16, 0.575 and 1.125 mg/mL. All the 3 extracts had antiviral actions against to PPRSV by inhibiting and killing roles,but just phragmites polysaccharide has the blocking role in highest safety concentration. And the way of extract acting in protection of the cell was as the same as the whole herb, indicate these extract are the major portion of the Chinese medicine herb.