CAJ | 학술논문

为分析影响白油佐剂免疫效果的主要因素，在前期研究基础上，参照国内大量进口白油GW1的馏分烃类组成及含量，制备出3批白油（ JH）。以免疫效果较好的国内白油（ GN1、GN2）和进口白油（ GW1、GW2）作参照，首先分析了白油样品的馏分烃类组成含量和正碳数分布数据；然后分别制备新城疫灭活疫苗进行免疫，检测免疫鸡血清抗体效价和药物残留情况。结果显示，白油样品杂质含量由高到低的顺序是GW2＞GN2＞GW1＞GN1＞JH2＞JH3＞JH1，免疫效果由高到低的顺序是JH2＞GW1＞JH1＞GN2＞GN1＞GW2＞JH3。试验表明，影响白油佐剂免疫效果的主要因素是总芳烃含量和重金属含量，其次是白油的运动黏度。
위분석영향백유좌제면역효과적주요인소，재전기연구기출상，삼조국내대량진구백유GW1적류분경류조성급함량，제비출3비백유（ JH）。이면역효과교호적국내백유（ GN1、GN2）화진구백유（ GW1、GW2）작삼조，수선분석료백유양품적류분경류조성함량화정탄수분포수거；연후분별제비신성역멸활역묘진행면역，검측면역계혈청항체효개화약물잔류정황。결과현시，백유양품잡질함량유고도저적순서시GW2＞GN2＞GW1＞GN1＞JH2＞JH3＞JH1，면역효과유고도저적순서시JH2＞GW1＞JH1＞GN2＞GN1＞GW2＞JH3。시험표명，영향백유좌제면역효과적주요인소시총방경함량화중금속함량，기차시백유적운동점도。
To analyze the influence factors of injection white oil adjuvant on immune effects, 3 batches of injection white oil ( JH) were prepared on basis of preliminary studies and by reference to large amount of the composition as well as the content of distillate hydrocarbon types in foreign GW1 white oil. Firstly we took the national injection white oil ( GN2, GN1 ) which had better immune effects and imports injection white oil ( GW1, GW2 ) as reference and analysed the content of distillate hydrocarbon types, data about carbon number distribution of injection white oil samples. Then inavtivated newcastle disease vaccine were prepared for immunization effect test to detect the serum antibody titer and the drug residues of the immune chicken. It turned out that the amount of impurityes in white oil samples ranged from high to low, the order was GW2>GN2>GW1>GN1>JH2>JH3>JH1. The immune effects order from high to weak was JH2> GW1> JH1> GN2> GN1> GW2> JH3. Experimental analysis concludes that the main factors affecting the white oil adjuvant effect are the content of total aromatic and the heavy mental concentrations, the kinematic viscosity of the white oil is less related.