CAJ | 학술논문

为提高重组蛋白的生产效率，利用响应面试验设计技术，对表达猪肺炎支原体P 46蛋白的重组大肠杆菌DE-pET-P46的培养基进行了优化，确定了培养基各营养成分及其最佳配比。在相同培养条件下，优化培养基比LB培养基的菌体浓度高出一倍。在生物反应器中利用优化培养基发酵培养该重组大肠杆菌，培养物的湿菌重达39．5 g/L。 SDS-PAGE电泳结果显示，高密度发酵对重组大肠杆菌DE-pET-P46的rP46的表达和占细胞总蛋白比例均没有明显的改变。
위제고중조단백적생산효솔，이용향응면시험설계기술，대표체저폐염지원체P 46단백적중조대장간균DE-pET-P46적배양기진행료우화，학정료배양기각영양성분급기최가배비。재상동배양조건하，우화배양기비LB배양기적균체농도고출일배。재생물반응기중이용우화배양기발효배양해중조대장간균，배양물적습균중체39．5 g/L。 SDS-PAGE전영결과현시，고밀도발효대중조대장간균DE-pET-P46적rP46적표체화점세포총단백비례균몰유명현적개변。
For higher efficiency and lower cost of the protein production, response surface analysis(RSA) was used for culture optimization of the recombinant E. coli which could express Mycoplasma hyopneumoniae P46 protein, and the best ratio of each medium component was determined. Under the same culture conditions, the cell concentration of the optimized culture is twice higher than LB medium. The medium used in a bioreactor for the recombinant E. coli DE-pET-P46 culture fermentation, the wet bacteria weight of the culture is up to 39.5 g/L. SDS-PAGE electrophoresis showed that there is no obvious difference on the rP46 expression of the recombinant E. coli DE-pET-P46 between cultivated with bioreactor and with shaking incubator.